Lagaaij E L, Ruigrok M B, van Rood J J, Hendriks G F, van der Woude F, Weimar W, van Houwelingen H C, Goulmy E
Department of Immunohaematology and Blood Bank, University Hospital Leiden, The Netherlands.
J Immunol. 1991 Nov 15;147(10):3348-52.
We have recently observed that the HLA-DR match between recipients and transfusion donors influences the beneficial effect of blood transfusions on allograft survival. To examine the immunologic effects of one-HLA-DR-Ag-matched and completely DR-mismatched transfusions, transfusion-induced changes in cell-mediated lympholysis (CML) were investigated. Blood donor directed CTL activity was measured in vitro before and after blood transfusion in 56 candidates for organ transplantation who received planned HLA-typed blood. We report that blood donor-directed CTL activity increased substantially after a single transfusion mismatched with the recipient for two HLA-DR Ag (p less than 0.0001). A transfusion matched for one HLA-DR Ag did not enhance CTL activity. No correlation was found between CTL reactivity and sharing of HLA class I Ag. The present study supports our previous observation that matching for at least one HLA class II Ag (HLA-DR) between transfusion recipient and blood donor is required if immunization by blood transfusion is to be avoided. These data show that the presence or absence of "autologous" HLA-DR Ag on the leucocytes of the transfusion donor plays a decisive rol whether immunization or immune suppression will ensue.
我们最近观察到，受者与输血供者之间的HLA - DR匹配会影响输血对同种异体移植物存活的有益作用。为了研究单HLA - DR抗原匹配和完全DR不匹配输血的免疫效应，我们调查了输血诱导的细胞介导淋巴细胞溶解（CML）的变化。在56名接受计划HLA分型输血的器官移植候选者中，在输血前后体外测量了供血者定向的CTL活性。我们报告，在与受者有两个HLA - DR抗原不匹配的单次输血后，供血者定向的CTL活性显著增加（p小于0.0001）。一个HLA - DR抗原匹配的输血并未增强CTL活性。未发现CTL反应性与HLAⅠ类抗原共享之间存在相关性。本研究支持我们先前的观察结果，即如果要避免输血免疫，输血受者与供血者之间至少需要一个HLAⅡ类抗原（HLA - DR）匹配。这些数据表明，输血供者白细胞上“自体”HLA - DR抗原的存在与否对是否会发生免疫或免疫抑制起着决定性作用。
