沙眼衣原体感染对自然流产的影响。

The influence of Chlamydia trachomatis infection on spontaneous abortions.

机构信息

Centre for STD Research and Diagnostics, Bialystok, Poland.

出版信息

Adv Med Sci. 2009;54(1):86-90. doi: 10.2478/v10039-009-0008-5.

DOI:10.2478/v10039-009-0008-5

PMID:19403438

Abstract

PURPOSE

The aim of the study was to evaluate the frequency of Chlamydia trachomatis (C.t.) infection among women who experienced a miscarriage.

MATERIALS AND METHODS

Patients referred to the Centre for STD Research and Diagnostics in Bialystok from the Department of Perinatology and from gynaecological outpatient clinics, after spontaneous abortion were enrolled in the study. C.t. infection diagnostics were performed among 76 women with 1 miscarriage and 44 patients with > or =2 miscarriages in anamnesis. Forty-six patients in the 2nd and the 3rd trimester of normal pregnancy served as a comparative group. Endocervical swabs as well as blood serum were obtained. To detect chlamydial DNA, direct PCR method was performed (Roche, Molecular Systems, N.J., USA). To detect IgA and IgG specific anti-chlamydial antibodies we used immunoenzymatic assay (medac, Hamburg, Germany).

RESULTS

In patients with 1 miscarriage (gr.1), C.t. infection by means of PCR was detected in 11.8% of women (p=0.029), in patients with > or =2 miscarriages (gr.2) in 9.1% (p=0.198) and in the comparative group (gr.0) in 2.2%. Specific anti-chlamydial antibodies IgA class were detected in: 7.9 (p=0.082) in group 1, 4.5% (p=0.236) in group 2 and in 0% in group 0, and IgG class in 21.1% (p=0.024), 36.4% (p=0.000) and in 4.4%, respectively.

CONCLUSIONS

C.t. infection is an important causative agent of miscarriages in women. 2. C.t. infection diagnostic procedures should be considered in screening tests during pregnancy.

摘要

目的

本研究旨在评估经历自然流产的女性中沙眼衣原体（C.t.）感染的频率。

材料和方法

将因自然流产从围产期学部和妇科门诊转介到比亚韦斯托克 STD 研究和诊断中心的患者纳入本研究。对 76 名有 1 次流产史的患者和 44 名有 >或=2 次流产史的患者进行 C.t. 感染诊断。46 名处于正常妊娠第 2 和第 3 trimester 的患者作为对照组。采集宫颈拭子和血清。使用直接 PCR 方法（Roche，Molecular Systems，N.J.，USA）检测沙眼衣原体 DNA。使用免疫酶联测定法（medac，Hamburg，Germany）检测 IgA 和 IgG 特异性抗沙眼衣原体抗体。

结果

在有 1 次流产史的患者（gr.1）中，PCR 法检测到 C.t. 感染的患者占 11.8%（p=0.029），在有 >或=2 次流产史的患者（gr.2）中占 9.1%（p=0.198），在对照组（gr.0）中占 2.2%。检测到 IgA 类特异性抗沙眼衣原体抗体：在 gr.1 组中为 7.9%（p=0.082），在 gr.2 组中为 4.5%（p=0.236），在 gr.0 组中为 0%，在 IgG 类中为 21.1%（p=0.024），在 gr.2 组中为 36.4%（p=0.000），在 gr.0 组中为 4.4%。