Tyagi Sanjay
Public Health Research Institute, New Jersey Medical School, University of Medicine & Dentistry of New Jersey, Newark, New Jersey, USA.
Nat Methods. 2009 May;6(5):331-8. doi: 10.1038/nmeth.1321.
Powerful methods now allow the imaging of specific mRNAs in living cells. These methods enlist fluorescent proteins to illuminate mRNAs, use labeled oligonucleotide probes and exploit aptamers that render organic dyes fluorescent. The intracellular dynamics of mRNA synthesis, transport and localization can be analyzed at higher temporal resolution with these methods than has been possible with traditional fixed-cell or biochemical approaches. These methods have also been adopted to visualize and track single mRNA molecules in real time. This review explores the promises and limitations of these methods.
如今，强大的技术方法能够对活细胞中的特定信使核糖核酸（mRNA）进行成像。这些方法利用荧光蛋白来照亮mRNA，使用标记的寡核苷酸探针，并利用能使有机染料发出荧光的适体。与传统的固定细胞或生化方法相比，利用这些方法可以以更高的时间分辨率分析mRNA合成、运输和定位的细胞内动态变化。这些方法还被用于实时可视化和追踪单个mRNA分子。本综述探讨了这些方法的前景与局限性。
