Santangelo Philip J, Lifland Aaron W, Curt Paul, Sasaki Yukio, Bassell Gary J, Lindquist Michael E, Crowe James E
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
Nat Methods. 2009 May;6(5):347-9. doi: 10.1038/nmeth.1316. Epub 2009 Apr 6.
To visualize native or non-engineered RNA in live cells with single-molecule sensitivity, we developed multiply labeled tetravalent RNA imaging probes (MTRIPs). When delivered with streptolysin O into living human epithelial cancer cells and primary chicken fibroblasts, MTRIPs allowed the accurate imaging of native mRNAs and a non-engineered viral RNA, of RNA co-localization with known RNA-binding proteins, and of RNA dynamics and interactions with stress granules.
为了以单分子灵敏度在活细胞中可视化天然或未经过工程改造的RNA，我们开发了多重标记的四价RNA成像探针（MTRIPs）。当与链球菌溶血素O一起导入活的人上皮癌细胞和原代鸡成纤维细胞时，MTRIPs能够对天然mRNA和一种未经过工程改造的病毒RNA进行精确成像，对RNA与已知RNA结合蛋白的共定位进行成像，以及对RNA动态变化和与应激颗粒的相互作用进行成像。
