Chung Kyungmi, Okabe Satoshi
Department of Urban and Environmental Engineering, Hokkaido University, Kita-ku, Sapporo, Japan.
Appl Microbiol Biotechnol. 2009 Jul;83(5):965-77. doi: 10.1007/s00253-009-1990-z. Epub 2009 Apr 29.
A mediator-less three-stage two-chamber microbial fuel cell (MFC) system was developed and operated continuously for more than 1.5 years to evaluate continuous power generation while treating artificial wastewater containing glucose (10 mM) concurrently. A stable power density of 28 W/m(3) was attained with an anode hydraulic retention time of 4.5 h and phosphate buffer as the cathode electrolyte. An overall dissolved organic carbon removal ratio was about 85%, and coulombic efficiency was about 46% in this MFC system. We also analyzed the microbial community structure of anode biofilms in each MFC. Since the environment in each MFC was different due to passing on the products to the next MFC in series, the microbial community structure was different accordingly. The anode biofilm in the first MFC consisted mainly of bacteria belonging to the Gammaproteobacteria, identified as Aeromonas sp., while the Firmicutes dominated the anode biofilms in the second and third MFCs that were mainly fed with acetate. Cyclic voltammetric results supported the presence of a redox compound(s) associated with the anode biofilm matrix, rather than mobile (dissolved) forms, which could be responsible for the electron transfer to the anode. Scanning electron microscopy revealed that the anode biofilms were comprised of morphologically different cells that were firmly attached on the anode surface and interconnected each other with anchor-like filamentous appendages, which might support the results of cyclic voltammetry.
开发了一种无介质的三阶段双室微生物燃料电池（MFC）系统，并连续运行超过1.5年，以评估其在同时处理含葡萄糖（10 mM）的人工废水时的连续发电情况。在阳极水力停留时间为4.5小时且以磷酸盐缓冲液作为阴极电解液的条件下，获得了28 W/m³的稳定功率密度。在此MFC系统中，总溶解有机碳去除率约为85%，库仑效率约为46%。我们还分析了每个MFC中阳极生物膜的微生物群落结构。由于每个MFC中的环境因将产物依次传递给下一个MFC而有所不同，微生物群落结构也相应不同。第一个MFC中的阳极生物膜主要由属于γ-变形菌纲的细菌组成，鉴定为气单胞菌属，而厚壁菌门在主要以乙酸盐为食的第二个和第三个MFC的阳极生物膜中占主导地位。循环伏安法结果支持存在与阳极生物膜基质相关的氧化还原化合物，而非可移动（溶解）形式，这可能是电子转移至阳极的原因。扫描电子显微镜显示，阳极生物膜由形态不同的细胞组成，这些细胞牢固地附着在阳极表面，并通过锚状丝状附属物相互连接，这可能支持了循环伏安法的结果。
