Ki D, Park J, Lee J, Yoo K
Department of Civil & Environmental Engineering, Yonsei University, 262 Sungsanno, Seodaemun-gu, Seoul, South Korea.
Water Sci Technol. 2008;58(11):2195-201. doi: 10.2166/wst.2008.577.
In this study, we performed microbial community analysis to examine microbial diversity and community structure in microbial fuel cells (MFCs) seeded with activated sludge from a municipal wastewater treatment plant in South Korea. Because anode-attached biofilm populations are particularly important in electricity transfer, the ecological characteristics of anode-attached biofilm microbes were explored and compared with those of microbes grown in suspension in an anode chamber. 16S rDNA-based community analysis showed that the degree of diversity in anode-attached biofilms was greater than that of the originally seeded activated sludge as well as that of the suspension-grown microbes in the anode bottle. In addition, Bacteroidetes and Clostridia grew preferentially during MFC electricity generation. Further phylogenetic analysis revealed that the anode biofilm populations described in this work are phylogenetically distant from previously characterized MFC anode biofilm microbes. These findings suggest that a phylogenetically diverse set of microbes can be involved in the electricity generation of MFC anode compartments, and that increased microbial diversity in anode biofilms may help to stabilize electricity production in the MFC.
在本研究中，我们进行了微生物群落分析，以检测接种韩国某城市污水处理厂活性污泥的微生物燃料电池（MFC）中的微生物多样性和群落结构。由于附着在阳极的生物膜菌群在电传递过程中尤为重要，因此我们探究了附着在阳极的生物膜微生物的生态特性，并将其与在阳极室中悬浮生长的微生物的生态特性进行了比较。基于16S rDNA的群落分析表明，附着在阳极的生物膜中的多样性程度高于最初接种的活性污泥以及阳极瓶中悬浮生长的微生物。此外，拟杆菌门和梭菌纲在MFC发电过程中优先生长。进一步的系统发育分析表明，本研究中描述的阳极生物膜菌群在系统发育上与先前表征的MFC阳极生物膜微生物相距甚远。这些发现表明，系统发育上多样的一组微生物可能参与MFC阳极室的发电过程，并且阳极生物膜中微生物多样性的增加可能有助于稳定MFC中的电力生产。
