Dessì Paolo, Porca Estefania, Haavisto Johanna, Lakaniemi Aino-Maija, Collins Gavin, Lens Piet N L
Laboratory of Chemistry and Bioengineering, Tampere University of Technology P.O. Box 541 FI-33101 Tampere Finland
Microbial Communities Laboratory, School of Natural Sciences, National University of Ireland Galway University Road Galway H91 TK33 Ireland.
RSC Adv. 2018 Jan 15;8(6):3069-3080. doi: 10.1039/c7ra12316g. eCollection 2018 Jan 12.
A mesophilic (37 °C) and a thermophilic (55 °C) two-chamber microbial fuel cell (MFC) were studied and compared for their power production from xylose and the microbial communities involved. The anode-attached, membrane-attached, and planktonic microbial communities, and their respective active subpopulations, were determined by next generation sequencing (Illumina MiSeq), based on the presence and expression of the 16S rRNA gene. accounted for 65% of the anode-attached active microbial community in the mesophilic MFC, and were associated to electricity generation likely through direct electron transfer, resulting in the highest power production of 1.1 W m. A lower maximum power was generated in the thermophilic MFC (0.2 W m), likely due to limited acetate oxidation and the competition for electrons by hydrogen oxidizing bacteria and hydrogenotrophic methanogenic archaea. Aerobic microorganisms, detected among the membrane-attached active community in both the mesophilic and thermophilic MFC, likely acted as a barrier for oxygen flowing from the cathodic chamber through the membrane, favoring the strictly anaerobic exoelectrogenic microorganisms, but competing with them for xylose and its degradation products. This study provides novel information on the active microbial communities populating the anodic chamber of mesophilic and thermophilic xylose-fed MFCs, which may help in developing strategies to favor exoelectrogenic microorganisms at the expenses of competing microorganisms.
研究并比较了中温（37°C）和高温（55°C）双室微生物燃料电池（MFC）利用木糖产电的情况以及所涉及的微生物群落。基于16S rRNA基因的存在和表达，通过下一代测序（Illumina MiSeq）确定了附着在阳极、附着在膜上以及浮游的微生物群落及其各自的活跃亚群。在中温MFC中， 占附着在阳极的活跃微生物群落的65%，可能通过直接电子转移与发电相关，从而产生了最高1.1 W/m的功率。高温MFC产生的最大功率较低（0.2 W/m），这可能是由于乙酸盐氧化受限以及氢氧化细菌和氢营养型产甲烷古菌对电子的竞争。在中温和高温MFC的附着在膜上的活跃群落中均检测到的需氧微生物，可能充当了氧气从阴极室通过膜流动的屏障，有利于严格厌氧的外生电微生物，但与它们竞争木糖及其降解产物。本研究提供了关于中温和高温木糖喂养的MFC阳极室中活跃微生物群落的新信息，这可能有助于制定以竞争微生物为代价来促进外生电微生物生长的策略。
