Department of Ichthyology & Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Nea Ionia, Greece.
Environ Monit Assess. 2010 Jun;165(1-4):27-38. doi: 10.1007/s10661-009-0924-7. Epub 2009 Apr 30.
We analyzed the bacterial 16S rRNA gene diversity throughout the major components of the drinking water distribution system of a ca. 52,000-inhabitants city (Trikala City, Greece) in order to describe the changes of the bacterial assemblages and to detect possible bacterial pathogens which are not included in the standard monitoring process. Bacterial DAPI counts and DNA extraction was performed in the water pumping wells, the water treatment tank and tap water from households. Approximately 920 bp of the bacterial 16S rDNA were PCR-amplified, cloned, and sequenced for a total of 191 clones, which belonged to 112 unique phylotypes. The water of the pumping wells harbored a typical subsurface bacterial assemblage, with no human pathogens, dominated by beta-Proteobacteria. Cell abundance in the water treatment tank decreased significantly, close to detection limit, but bacterial diversity remained high. However, the dominance of beta-Proteobacteria decreased considerably, indicating the sensitivity of this group to drinking water disinfection treatment. Tap water from the households hosted a much less diverse, low-cell bacterial assemblage, dominated by Mycobacterium-like phylotypes, related to biofilm bacterial communities.
我们分析了一个约 52000 居民城市（希腊特里卡拉市）饮用水分配系统主要组成部分的细菌 16S rRNA 基因多样性，以便描述细菌群落的变化，并检测可能未包含在标准监测过程中的细菌病原体。在水泵井、水处理罐和家庭自来水中进行细菌 DAPI 计数和 DNA 提取。总共对 191 个克隆进行了约 920 bp 的细菌 16S rDNA 的 PCR 扩增、克隆和测序，这些克隆属于 112 个独特的类群。水泵井中的水携带典型的地下细菌群落，没有人类病原体，主要是β变形菌。水处理罐中的细胞丰度显著下降，接近检测限，但细菌多样性仍然很高。然而，β变形菌的优势显著下降，表明该组对饮用水消毒处理敏感。家庭自来水中的细菌群落多样性较低，细胞数量较少，以类似分枝杆菌的类群为主，与生物膜细菌群落有关。
