Baffoe Mavis, Koffuor George, Baffour-Awuah Agyapong, Sallah Lorraine
Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Department of Physiology, School of Medicine and Dentistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
J Exp Pharmacol. 2021 Mar 4;13:223-234. doi: 10.2147/JEP.S283557. eCollection 2021.
Male sexual dysfunction negatively affects an individual's quality of life and thus its of prime public concern, hence the need to boost reproductive abilities in such individuals. This study assessed the effect of hydroethanolic root extracts of (CBRE), (SJRE), and (PPRE), commonly used as aphrodisiacs in Ghana, using male Sprague-Dawley rats.
Plasma testosterone, follicle-stimulating hormone, and luteinizing hormone were assayed in grouped rats treated orally with 1 mL/kg normal saline, 50 mg/kg monosodium glutamate (MSG), and 100, 300, or 1000 mg/kg CBRE, SJRE, and PPRE, respectively, for 60 days. Epididymis and testis weights were determined. Semen was assessed on spermatozoa count, motility, and morphology. Malonyladehyde formation in lipid-peroxidation assay and histological examinations were performed to assess pathological changes in testes. Testicular testosterone was also assayed.
While MSG, CBRE, SJRE, and PPRE treatments did not result in significant reduction (>0.05) in plasma testosterone, there was significant reduction (≤0.05 -0.0001) in plasma luteinizing hormone, and follicle-stimulating hormone. The combined mean wet weights of epididymides and testes of all treated groups did not vary significantly (>0.05) from the control. There was significant reduction (≤0.0001) in sperm motility and count, with significant morphological changes (≤0.05-0.001), ie, bent necks, tails, and midpieces, and multiple anomalies in the spermatozoa in extract and MSG-treated groups. There was also significant (≤0.0001) reduction in testicular testosterone among all treatment groups.
Hydroethanolic CBRE, SJRE, and PPRE were found to have detrimental effects on reproductive function with prolonged usage and thus may not be safe to use in healthy males who intend to reproduce.
男性性功能障碍会对个人生活质量产生负面影响，因此备受公众关注，所以有必要提高这类人群的生殖能力。本研究使用雄性斯普拉格-道利大鼠，评估了加纳常用作壮阳药的可乐果（CBRE）、刺蒺藜（SJRE）和非洲李（PPRE）的水乙醇根提取物的效果。
将大鼠分组，分别口服1 mL/kg生理盐水、50 mg/kg味精（MSG）以及100、300或1000 mg/kg的CBRE、SJRE和PPRE，持续60天，之后测定血浆睾酮、促卵泡激素和促黄体生成素。测定附睾和睾丸重量。评估精液的精子数量、活力和形态。进行脂质过氧化测定中的丙二醛形成检测以及组织学检查，以评估睾丸的病理变化。同时也测定睾丸睾酮。
虽然MSG、CBRE、SJRE和PPRE处理并未导致血浆睾酮显著降低（>0.05），但血浆促黄体生成素和促卵泡激素显著降低（≤0.05 - 0.0001）。所有处理组附睾和睾丸的合并平均湿重与对照组相比无显著差异（>0.05）。提取物和MSG处理组的精子活力和数量显著降低（≤0.0001），精子形态有显著变化（≤0.05 - 0.001），即颈部、尾部和中段弯曲，精子出现多种异常。所有处理组的睾丸睾酮也显著降低（≤0.0001）。
发现长期使用水乙醇CBRE、SJRE和PPRE对生殖功能有不利影响，因此对于打算生育的健康男性而言，使用这些提取物可能不安全。
