Harada Takunori, Miyoshi Yuichi, Kuroda Reiko
ERATO-SORST Kuroda Chiromorphology Team, Japan Science and Technology Agency, 4-7-6 Komaba, Meguro-ku, Tokyo, Japan.
Rev Sci Instrum. 2009 Apr;80(4):046101. doi: 10.1063/1.3098945.
A new dual-purpose transmittance circular dichroism (CD) and diffuse reflectance CD (DRCD) universal chiroptical spectrophotometer (UCS-3, J-800KCMFII) was successfully developed to have the capability of measuring DRCD spectra down to 190 nm with high efficiency, based on UCS-2: J-800KCMF. Optical components newly used in UCS-3 are the integrating sphere of optimum size and material to achieve high performance particularly in the shorter wavelength region, a baffle installed to reduce the first specular reflection signals, and a condenser lens to increase light intensity per area for small samples. As a result, UCS-3 has become a very powerful instrument to measure DRCD spectra of powdered samples in situ, with approximately 20 times sensitivity of existing UCS-2. We could show that to achieve similar quality DRCD spectra, only 50 mug of (S)-(+)-1,1(')-binaphthyl-2,2(')-diyl hydrogen phosphate is required on UCS-3, as compared with 1.12 mg for UCS-2.
基于UCS-2：J-800KCMF，成功开发出一种新型的双用途透光圆二色（CD）和漫反射CD（DRCD）通用手性光学分光光度计（UCS-3，J-800KCMFII），它能够高效测量低至190nm的DRCD光谱。UCS-3中新使用的光学组件包括：尺寸和材料最佳的积分球，以在较短波长区域实现高性能；安装的挡板，用于减少首次镜面反射信号；以及聚光镜，用于增加小样品的单位面积光强。结果，UCS-3成为一种非常强大的仪器，可原位测量粉末样品的DRCD光谱，其灵敏度约为现有UCS-2的20倍。我们可以证明，为了获得质量相似的DRCD光谱，UCS-3仅需要50μg的（S）-（+）-1,1'-联萘-2,2'-二磷酸氢酯，而UCS-2则需要1.12mg。
