Zhuo Shuangmu, Chen Jianxin, Xie Shusen, Hong Zhibin, Jiang Xingshan
J Biomed Opt. 2009 Mar-Apr;14(2):020503. doi: 10.1117/1.3088029.
Intrinsic two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) signals are shown to differentiate between normal and neoplastic human esophageal stroma. It was found that TPEF and SHG signals from normal and neoplastic stroma exhibit different organization features, providing quantitative information about the biomorphology and biochemistry of tissue. By comparing normal with neoplastic stroma, there were significant differences in collagen-related changes, elastin-related changes, and alteration in proportions of matrix molecules, giving insight into the stromal changes associated with cancer progression and providing substantial potential to be applied in vivo to the clinical diagnosis of epithelial precancers and cancers.
研究表明，固有双光子激发荧光（TPEF）和二次谐波产生（SHG）信号可区分正常和肿瘤性人类食管基质。研究发现，正常和肿瘤性基质的TPEF和SHG信号呈现出不同的组织特征，提供了有关组织生物形态学和生物化学的定量信息。通过比较正常基质和肿瘤性基质，在胶原相关变化、弹性蛋白相关变化以及基质分子比例改变方面存在显著差异，这有助于深入了解与癌症进展相关的基质变化，并为体内应用于上皮性癌前病变和癌症的临床诊断提供了巨大潜力。
