激发波长对混浊介质非线性光学显微镜中穿透深度的影响。
Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media.
作者信息
Balu Mihaela, Baldacchini Tommaso, Carter John, Krasieva Tatiana B, Zadoyan Ruben, Tromberg Bruce J
机构信息
University of California, Irvine, Beckman Laser Institute Laser Microbeam and Medical Program, Irvine, CA 92617, USA.
出版信息
J Biomed Opt. 2009 Jan-Feb;14(1):010508. doi: 10.1117/1.3081544.
Abstract
We present a comparative study of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) imaging in turbid media at 800- and 1300-nm excitation. The depth-dependent decay of TPEF and SHG signals in turbid tissue phantoms is used to estimate the impact of light scattering on excitation intensity at each wavelength. A 50 to 80% increase in scattering length is observed using 1300-nm excitation, while peak TPEF emission intensity is obtained 10 to 20 microm beneath the surface for both sources. The increased penetration depth at 1300 nm is confirmed by TPEF and SHG microscopy of tissue phantoms composed of gelatin/microspheres and 3-D organotypic collagen-fibroblast cultures, respectively. Our results establish the feasibility of 1.3-microm excitation in nonlinear optical microscopy.
摘要
我们展示了在800纳米和1300纳米激发下，对浑浊介质中的双光子激发荧光（TPEF）和二次谐波产生（SHG）成像的比较研究。浑浊组织模型中TPEF和SHG信号随深度的衰减用于估计光散射对每个波长激发强度的影响。使用1300纳米激发时，观察到散射长度增加了50%至80%，而两种光源在表面下方10至20微米处均获得了TPEF发射峰值强度。分别由明胶/微球和三维器官型胶原-成纤维细胞培养物组成的组织模型的TPEF和SHG显微镜检查证实了1300纳米处穿透深度的增加。我们的结果确立了非线性光学显微镜中1.3微米激发的可行性。
相似文献
1
Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media.激发波长对混浊介质非线性光学显微镜中穿透深度的影响。
J Biomed Opt. 2009 Jan-Feb;14(1):010508. doi: 10.1117/1.3081544.
2
Measuring the scattering coefficient of turbid media from two-photon microscopy.通过双光子显微镜测量混浊介质的散射系数。
Opt Express. 2013 Oct 21;21(21):25221-35. doi: 10.1364/OE.21.025221.
3
Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy.脉冲持续时间对非线性光学显微镜中双光子激发荧光和二次谐波产生的影响。
J Biomed Opt. 2006 Mar-Apr;11(2):020501. doi: 10.1117/1.2177676.
4
Hybrid reflecting objectives for functional multiphoton microscopy in turbid media.用于浑浊介质中功能多光子显微镜的混合反射物镜
Opt Lett. 2006 Aug 15;31(16):2447-9. doi: 10.1364/ol.31.002447.
5
Two-photon excitation fluorescence microscopy with a high depth of field using an axicon.使用轴棱锥的具有高景深的双光子激发荧光显微镜。
Appl Opt. 2006 Dec 20;45(36):9246-52. doi: 10.1364/ao.45.009246.
6
Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium.表征混浊介质中双光子荧光显微镜的点扩散函数。
J Biomed Opt. 2003 Jul;8(3):450-9. doi: 10.1117/1.1578644.
7
Imaging through a scattering medium with an interferential spectrometer by selection of an amplitude modulation correlator.通过选择幅度调制相关器，利用干涉光谱仪对散射介质进行成像。
Opt Lett. 2004 Dec 15;29(24):2908-10. doi: 10.1364/ol.29.002908.
8
Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source.使用12飞秒宽带光源的联合多光子显微镜和光学相干断层扫描技术。
J Biomed Opt. 2006 Mar-Apr;11(2):020502. doi: 10.1117/1.2193428.
9
Enhancement of imaging depth of two-photon microscopy using pinholes: analytical simulation and experiments.使用针孔增强双光子显微镜的成像深度：分析模拟与实验
Opt Express. 2012 Aug 27;20(18):20605-22. doi: 10.1364/OE.20.020605.
10
Forty-five degree backscattering-mode nonlinear absorption imaging in turbid media.混浊介质中四十五度背散射模式非线性吸收成像。
J Biomed Opt. 2010 Mar-Apr;15(2):026004. doi: 10.1117/1.3368995.
引用本文的文献
1
Window into the Brain: In Vivo Multiphoton Imaging.大脑之窗：活体多光子成像
ACS Photonics. 2024 Dec 24;12(1):1-15. doi: 10.1021/acsphotonics.4c00958. eCollection 2025 Jan 15.
2
Lanthanide ion-doped upconversion nanoparticles for low-energy super-resolution applications.用于低能量超分辨率应用的镧系离子掺杂上转换纳米粒子。
Light Sci Appl. 2024 Sep 14;13(1):252. doi: 10.1038/s41377-024-01547-6.
3
Probing metalloenzyme dynamics in living systems: Contemporary advances in fluorescence imaging tools and applications.探索生物系统中的金属酶动力学：荧光成像工具及其应用的当代进展
Curr Opin Chem Biol. 2024 Aug;81:102475. doi: 10.1016/j.cbpa.2024.102475. Epub 2024 Jun 8.
4
mtFRC: depth-dependent resolution quantification of image features in 3D fluorescence microscopy.mtFRC：三维荧光显微镜中图像特征的深度相关分辨率量化
Bioinform Adv. 2023 Dec 18;3(1):vbad182. doi: 10.1093/bioadv/vbad182. eCollection 2023.
5
Chemical Reactions in Living Systems.生命体系中的化学反应。
Adv Sci (Weinh). 2024 Feb;11(8):e2303396. doi: 10.1002/advs.202303396. Epub 2023 Sep 7.
6
Challenges and opportunities for SERS in the infrared: materials and methods.表面增强拉曼光谱在红外波段的挑战与机遇：材料与方法
Nanoscale Adv. 2023 Mar 22;5(8):2132-2166. doi: 10.1039/d2na00930g. eCollection 2023 Apr 11.
7
Multiphoton microscopy for label-free multicolor imaging of peripheral nerve.多光子显微镜用于周围神经的无标记多色成像。
J Biomed Opt. 2022 May;27(5). doi: 10.1117/1.JBO.27.5.056501.
8
Multiphoton excitation imaging via an actively mode-locked tunable fiber-cavity SOA laser around 800 nm.通过围绕800纳米的主动锁模可调谐光纤腔半导体光放大器激光器进行多光子激发成像。
Biomed Opt Express. 2022 Jan 3;13(2):525-538. doi: 10.1364/BOE.447010. eCollection 2022 Feb 1.
9
Fast, large area multiphoton exoscope (FLAME) for macroscopic imaging with microscopic resolution of human skin.快速、大面积多光子外照镜（FLAME），用于实现人类皮肤的宏观成像与微观分辨率。
Sci Rep. 2020 Oct 22;10(1):18093. doi: 10.1038/s41598-020-75172-9.
10
Watt-level all-fiber optical parametric chirped-pulse amplifier working at 1300 nm.工作在1300纳米的瓦级全光纤光参量啁啾脉冲放大器。
Opt Lett. 2019 Jul 15;44(14):3422-3425. doi: 10.1364/OL.44.003422.
本文引用的文献
1
Influence of optical properties on two-photon fluorescence imaging in turbid samples.光学性质对浑浊样品中双光子荧光成像的影响。
Appl Opt. 2000 Mar 1;39(7):1194-201. doi: 10.1364/ao.39.001194.
2
Multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser.基于飞秒铬：镁橄榄石激光器的多模态非线性光谱显微镜。
Opt Lett. 2001 Dec 1;26(23):1909-11. doi: 10.1364/ol.26.001909.
3
Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy.脉冲持续时间对非线性光学显微镜中双光子激发荧光和二次谐波产生的影响。
J Biomed Opt. 2006 Mar-Apr;11(2):020501. doi: 10.1117/1.2177676.
4
MPScope: a versatile software suite for multiphoton microscopy.MPScope：一款用于多光子显微镜的多功能软件套件。
J Neurosci Methods. 2006 Sep 30;156(1-2):351-9. doi: 10.1016/j.jneumeth.2006.03.001. Epub 2006 Apr 18.
5
Photodynamic therapy on keloid fibroblasts in tissue-engineered keratinocyte-fibroblast co-culture.组织工程化角质形成细胞-成纤维细胞共培养体系中瘢痕疙瘩成纤维细胞的光动力疗法
Lasers Surg Med. 2005 Sep;37(3):231-44. doi: 10.1002/lsm.20213.
6
Two-photon imaging to a depth of 1000 microm in living brains by use of a Ti:Al2O3 regenerative amplifier.使用钛宝石再生放大器对活体大脑进行深度达1000微米的双光子成像。
Opt Lett. 2003 Jun 15;28(12):1022-4. doi: 10.1364/ol.28.001022.
7
Epifluorescence collection in two-photon microscopy.双光子显微镜中的落射荧光采集。
Appl Opt. 2002 Sep 1;41(25):5376-82. doi: 10.1364/ao.41.005376.
8
Two-photon microscopy in brain tissue: parameters influencing the imaging depth.脑组织中的双光子显微镜：影响成像深度的参数
J Neurosci Methods. 2001 Oct 15;111(1):29-37. doi: 10.1016/s0165-0270(01)00438-1.
Zotero 插件