An Dong-Shan, Yin Cheng-Ri, Lee Sung-Taik, Cho Chun-Hwi
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):1122-5. doi: 10.1099/ijs.0.003384-0.
A novel strain, designated Jip 10(T), isolated from dried rice straw, was characterized by a polyphasic approach to clarify its taxonomic position. The isolate was Gram-negative, facultatively aerobic, heterotrophic, non-motile, non-spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate clustered with the genus Mucilaginibacter in the phylum Bacteroidetes. 16S rRNA gene sequence similarities between strain Jip 10(T) and the type strains of Mucilaginibacter gracilis and Mucilaginibacter paludis were 93.7 and 93.6 %, respectively. The G+C content of the genomic DNA was 48.1 mol%. Chemotaxonomic data [major menaquinone MK-7 and major fatty acids iso-C(15 : 0), iso-C(17 : 0) 3-OH and summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c)] supported the affiliation of strain Jip 10(T) to the genus Mucilaginibacter. However, the results of physiological and biochemical tests allowed phenotypic differentiation of strain Jip 10(T) from other Mucilaginibacter species with validly published names. Therefore, strain Jip 10(T) (=KCTC 12639(T) =LMG 23488(T)) was classified in the genus Mucilaginibacter as the type strain of a novel species, for which the name Mucilaginibacter daejeonensis sp. nov. is proposed.
从干稻草中分离出的一株新菌株，命名为Jip 10(T)，采用多相分类法对其进行表征以明确其分类地位。该菌株为革兰氏阴性、兼性需氧、异养、不运动、不产芽孢且呈杆状。基于16S rRNA基因序列的系统发育分析表明，该菌株与拟杆菌门中的黏液杆菌属聚类在一起。菌株Jip 10(T)与纤细黏液杆菌和沼泽黏液杆菌模式菌株的16S rRNA基因序列相似性分别为93.7%和93.6%。基因组DNA的G+C含量为48.1 mol%。化学分类数据[主要甲基萘醌MK-7以及主要脂肪酸异-C(15 : 0)、异-C(17 : 0) 3-OH和总和特征3（异-C(15 : 0) 2-OH和/或C(16 : 1)ω7c）]支持菌株Jip 10(T)属于黏液杆菌属。然而，生理生化测试结果使菌株Jip 10(T)能够与其他已有效发表名称的黏液杆菌属物种在表型上区分开来。因此，菌株Jip 10(T)（=KCTC 12639(T) =LMG 23488(T)）被归入黏液杆菌属，作为一个新物种的模式菌株，为此提议将其命名为大田黏液杆菌（Mucilaginibacter daejeonensis sp. nov.）。
