Jeon Yeji, Lee Sang-Suk, Chung Bok Sil, Kim Jeong Myeong, Bae Jin-Woo, Park Soo Kwon, Jeon Che Ok
Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Jun;59(Pt 6):1451-4. doi: 10.1099/ijs.0.007138-0.
A Gram-negative-staining, non-spore-forming bacterium devoid of flagella, designated strain B9(T), was isolated from rice paddy soil associated with the roots of Oryza sativa collected from Jinju, South Korea. Cells were straight rods, were catalase- and oxidase-positive and were able to hydrolyse pectin, xylan and laminarin. Growth of strain B9(T) was observed between 15 and 35 degrees C (optimum 25-30 degrees C) and between pH 5.0 and 8.0 (optimum pH 6.5-7.5). Strain B9(T) contained menaquinone-7 (MK-7) as a major isoprenoid quinone and summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH), iso-C(15 : 0) and C(16 : 0) as major fatty acids. The G+C content of the genomic DNA was 44.4 mol%. Comparative 16S rRNA gene sequence analysis showed that strain B9(T) belonged to the genus Mucilaginibacter, a member of the family Sphingobacteriaceae, and was most closely related to Mucilaginibacter kameinonensis SCK(T) (95.9 % sequence similarity). On the basis of chemotaxonomic data and molecular properties, strain B9(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter oryzae sp. nov. is proposed. The type strain is B9(T) (=KACC 12816(T) =DSM 19975(T)).
从韩国晋州采集的与水稻根系相关的稻田土壤中分离出一株革兰氏阴性、不产芽孢、无鞭毛的细菌，命名为菌株B9(T)。细胞呈直杆状，过氧化氢酶和氧化酶呈阳性，能够水解果胶、木聚糖和海带多糖。菌株B9(T)在15至35℃（最适温度25 - 30℃）和pH 5.0至8.0（最适pH 6.5 - 7.5）之间生长。菌株B9(T)含有甲基萘醌-7（MK-7）作为主要类异戊二烯醌，以及特征性总和3（C(16 : 1)ω7c和/或异-C(15 : 0) 2-OH）、异-C(15 : 0)和C(16 : 0)作为主要脂肪酸。基因组DNA的G+C含量为44.4 mol%。比较16S rRNA基因序列分析表明，菌株B9(T)属于鞘脂杆菌科的粘杆菌属，与龟井粘杆菌SCK(T)关系最密切（序列相似性为95.9%）。基于化学分类数据和分子特性，菌株B9(T)代表粘杆菌属的一个新物种，为此提出新名称粘稻粘杆菌。模式菌株为B9(T)（=KACC 12816(T) =DSM 19975(T)）。
