Goldberg A J, Advincula M C, Komabayashi T, Patel P A, Mather P T, Goberman D G, Kazemi R B
Center for Biomaterials, Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT06030-1615, USA.
J Dent Res. 2009 Apr;88(4):377-81. doi: 10.1177/0022034509333838.
In situ formation of mineral particles by biocatalysis would be advantageous for occluding dentin tubules to reduce permeability or for sealing of material-tooth interfaces. One approach would require that the peptide-catalyst remain functional on the dentin surface. Based on recent observations of retained activity on other surfaces, we hypothesized that poly(L-lysine) (PLL), an analog of the protein catalyst responsible for silica formation in primitive marine species, would remain functional on dentin. PLL was applied to dentin discs along with a pre-hydrolyzed silica precursor, tetramethyl orthosilicate (TMOS). Discs were analyzed microscopically (scanning electron microscopy, SEM) and chemically (x-ray photoelectron spectroscopy, XPS). The treated discs, but not the negative controls, exhibited partial distinct coating whose XPS survey was consistent with that of silica, demonstrating that the polypeptide was required and retained its mediating activity. Peptide-catalysts that mediate mineral formation can retain functionality on dentin, suggesting a wide range of preventive and treatment strategies.
通过生物催化原位形成矿物颗粒，对于封闭牙本质小管以降低渗透性或密封材料与牙齿的界面是有利的。一种方法要求肽催化剂在牙本质表面保持活性。基于最近在其他表面上保留活性的观察结果，我们推测聚（L-赖氨酸）（PLL），一种在原始海洋物种中负责二氧化硅形成的蛋白质催化剂的类似物，在牙本质上仍将保持活性。将PLL与预水解的二氧化硅前体原硅酸四甲酯（TMOS）一起应用于牙本质圆盘。通过显微镜（扫描电子显微镜，SEM）和化学方法（X射线光电子能谱，XPS）对圆盘进行分析。经处理的圆盘，而非阴性对照，呈现出部分明显的涂层，其XPS检测结果与二氧化硅一致，表明该多肽是必需的且保留了其中介活性。介导矿物形成的肽催化剂可以在牙本质上保留功能，这提示了广泛的预防和治疗策略。
