通过单引物序列非依赖扩增法在从一次胃肠炎暴发中采集的多个样本中检测TT病毒。

Detection of TT virus by single-primer sequence-independent amplification in multiple samples collected from an outbreak of gastroenteritis.

作者信息

Braham S, Iturriza-Gómara M, Gray J

机构信息

Enteric Virus Unit, Virus Reference Department, Centre for Infections, Health Protection Agency, Colindale, London, NW9 5HT, UK.

出版信息

Arch Virol. 2009;154(6):981-5. doi: 10.1007/s00705-009-0387-8. Epub 2009 May 1.

DOI:10.1007/s00705-009-0387-8

PMID:19407928

Abstract

A panel of gastroenteritis outbreak samples was subjected to a virus purification and concentration algorithm followed by a sequence-independent amplification method devised to detect viral enteric pathogens. The application of these methods allowed the identification of torque teno virus (TTV) in one outbreak. The complete genome sequence of 3,260 nt was obtained through "genome walking", and four open reading frames were deduced from the genomic sequence. Phylogenetic analysis grouped this virus in TTV genetic group 3, clustering with genotype 27, with 85% similarity at the nt level with strain SAa-01.

摘要

一组肠胃炎暴发样本经过病毒纯化和浓缩算法处理，随后采用一种为检测肠道病毒病原体而设计的非序列依赖性扩增方法。这些方法的应用使得在一次暴发中鉴定出了细小病毒（TTV）。通过“基因组步移”获得了3260 nt的完整基因组序列，并从该基因组序列中推导了四个开放阅读框。系统发育分析将该病毒归类为TTV遗传组3，与27基因型聚类，在核苷酸水平上与SAa - 01菌株的相似性为85%。