玉米秸秆固定化细胞连续生产丙酮-丁醇-乙醇
Continuous acetone-butanol-ethanol production by corn stalk immobilized cells.
作者信息
Zhang Yuedong, Ma Yujiu, Yang Fangxiao, Zhang Chunhui
机构信息
Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266071 Qingdao, China.
出版信息
J Ind Microbiol Biotechnol. 2009 Aug;36(8):1117-21. doi: 10.1007/s10295-009-0582-3. Epub 2009 May 1.
Corn stalk was used as a support to immobilize Clostridia beijerinckii ATCC 55025 in the fermentation process of acetone, butanol, and ethanol production. The effect of the dilution rate on solvent production was examined in a steady-state 20-day continuous flow operation. The maximum total solvent concentration of 8.99 g l(-1) was obtained at a dilution rate of 0.2 h(-1). Increasing the dilution rate between 0.2 and 1.0 h(-1) resulted in an increased solvent productivity, and the highest solvent productivity was obtained at 5.06 g l(-1) h(-1) with a dilution rate of 1 h(-1). The maximum solvent yield from glucose of 0.32 g g(-1) was observed at 0.25 h(-1). The cell adsorption and morphology change during the growth on corn stalk support were examined by the SEM.
在丙酮、丁醇和乙醇生产的发酵过程中，玉米秸秆被用作载体来固定拜氏梭菌ATCC 55025。在为期20天的稳态连续流操作中，考察了稀释率对溶剂生产的影响。在稀释率为0.2 h⁻¹时，获得了最大总溶剂浓度8.99 g l⁻¹。将稀释率提高到0.2至1.0 h⁻¹之间会导致溶剂生产率提高，在稀释率为1 h⁻¹时，以5.06 g l⁻¹ h⁻¹获得了最高的溶剂生产率。在0.25 h⁻¹时，观察到葡萄糖的最大溶剂产率为0.32 g g⁻¹。通过扫描电子显微镜（SEM）研究了在玉米秸秆载体上生长过程中的细胞吸附和形态变化。
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