Campos Edhilvia J, Qureshi Nasib, Blaschek Hans P
University of Illinois, Biotechnology & Bioengineering Group, Department of Food Science & Human Nutrition, Urbana 61801, USA.
Appl Biochem Biotechnol. 2002 Spring;98-100:553-61. doi: 10.1385/abab:98-100:1-9:553.
In this article we report on acetone butanol ethanol (ABE) fermentation characteristics of degermed corn when using Clostridium beijerinckii BA101. Recent economic studies suggested that recovery of germ from corn and hence corn oil would help to make the ABE fermentation process more economical. C. beijerinckii BA101 ferments corn mash efficiently to produce ABE under appropriate nutritional and environmental conditions. Corn mash contains germ/corn oil that is, possibly, ancillary to the production of butanol during the ABE fermentation process. Since the presence of corn oil is not a critical factor in solvent fermentation, it can be removed and this will allow for byproduct credit. Batch fermentation of degermed corn resulted in 8.93 g/L of total ABE production as compared with 24.80 g/L of total ABE when supplemented with P2 medium nutrients. During the course of the germ separation process, corn steeping is required prior to grinding and removing the germ. It is likely that some nutrients from the corn are leached out during the steeping process. This may reduce the rate of fermentation and impact the final concentration of butanol/ABE that can be achieved. Fermentation of degermed corn with corn steep liquor resulted in the production of 19.28 g/L of ABE.
在本文中，我们报告了使用拜氏梭菌BA101时脱胚玉米的丙酮-丁醇-乙醇（ABE）发酵特性。最近的经济研究表明，从玉米中回收胚芽以及玉米油，将有助于使ABE发酵过程更具经济性。在适当的营养和环境条件下，拜氏梭菌BA101能有效地发酵玉米醪以生产ABE。玉米醪含有胚芽/玉米油，在ABE发酵过程中，这可能对丁醇的生产起到辅助作用。由于玉米油的存在不是溶剂发酵的关键因素，可以将其去除，这样就能获得副产品收益。与添加P2培养基营养成分时总ABE产量为24.80 g/L相比，脱胚玉米的分批发酵产生了8.93 g/L的总ABE。在胚芽分离过程中，研磨和去除胚芽之前需要进行玉米浸泡。在浸泡过程中，玉米中的一些营养成分可能会被浸出。这可能会降低发酵速率，并影响最终可达到的丁醇/ABE浓度。用玉米浸泡液对脱胚玉米进行发酵，产生了19.28 g/L的ABE。
