Zhang Wenjuan, Ji Weidong, Yang Linqing, Yang Jianping
School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
Wei Sheng Yan Jiu. 2009 Mar;38(2):139-43.
To study both cellular replicative and prematuce senescence induced by hydrogen peroxide (H2O2) of human embryonic lung fibroblasts (HEFs).
HEFs were cultured conventionally and treated with H2O2. Biomarkers of senescence were assessed, including cell morphology, growth curve, life span, distribution of cell cycle and senescence associated beta-galactosidase (SA-beta-gal), and the expression of proliferating cell nuclear antigen (PCNA).
HEFs entered replicative senescence status at 52 population doubling levels (PDL) and 400 micromol/L H2O2 induced premature senescence of HEFs in a short period. Senescent cells were characterized as enlarged, flattened and reduced saturation density, retarded in the G1 phase with increased expression of SA-beta-gal. The mRNA and protein level of PCNA decreased gradually during cellular senescence.
Cellular premature senescence could be induced by 400 micromol/L H2O2. Premature senescence induced by H2O2 could share the morphology characteristics and proliferative capacity similar to those of replicative one.
研究过氧化氢（H2O2）诱导人胚肺成纤维细胞（HEFs）发生细胞复制性衰老和早衰的情况。
常规培养HEFs并用H2O2处理。评估衰老的生物标志物，包括细胞形态、生长曲线、寿命、细胞周期分布和衰老相关β-半乳糖苷酶（SA-β-gal），以及增殖细胞核抗原（PCNA）的表达。
HEFs在52个群体倍增水平（PDL）时进入复制性衰老状态，400μmol/L H2O2可在短时间内诱导HEFs早衰。衰老细胞的特征为体积增大、扁平且饱和密度降低，在G1期阻滞，SA-β-gal表达增加。PCNA的mRNA和蛋白质水平在细胞衰老过程中逐渐降低。
400μmol/L H2O2可诱导细胞早衰。H2O2诱导的早衰与复制性衰老具有相似的形态特征和增殖能力。
