Wang Zhe, Wei Dandan, Xiao Hengyi
Department of Geriatrics, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
Methods Mol Biol. 2013;1048:135-44. doi: 10.1007/978-1-62703-556-9_11.
Normal somatic cells do not divide indefinitely and have their finite replicative lifespan. This property leads to an eventual arrest of cell division termed cell senescence. Human diploid fibroblasts offer a typical model for studying cell senescence in vitro. Various approaches to evoke oxidative stresses, such as the exposures of cells to ultraviolet light, ethanol, tert-butyl hydroperoxide (t-BHP), and peroxide hydrogen (H2O2), have been used to study the onset of cellular senescence. The early onset of cellular senescence induced by these stresses is termed stress-induced premature senescence (SIPS). In this manuscript, we will mainly summarize the basic knowledge and experimental approaches important for the induction of SIPS by H2O2, since H2O2 is the most commonly used inducer of SIPS in vitro and an endogenous source of cellular oxidative stress. Several assays methods generally used for testifying cell senescence are introduced.
正常体细胞不会无限分裂，具有有限的复制寿命。这种特性导致细胞分裂最终停滞，即细胞衰老。人二倍体成纤维细胞为体外研究细胞衰老提供了一个典型模型。已经采用了各种引发氧化应激的方法，如将细胞暴露于紫外线、乙醇、叔丁基过氧化氢（t-BHP）和过氧化氢（H2O2）中，来研究细胞衰老的起始。由这些应激诱导的细胞衰老早期起始被称为应激诱导的过早衰老（SIPS）。在本手稿中，我们将主要总结对于H2O2诱导SIPS重要的基础知识和实验方法，因为H2O2是体外最常用的SIPS诱导剂，也是细胞氧化应激的内源性来源。还介绍了几种通常用于验证细胞衰老的检测方法。
