Fathi Z, Dyster L M, Seto J, Condit R C, Niles E G
Department of Biochemistry, State University of New York, Buffalo 14214.
J Gen Virol. 1991 Nov;72 ( Pt 11):2733-7. doi: 10.1099/0022-1317-72-11-2733.
The frequency of recombination for a complete set of two-factor crosses between vaccinia virus mutations separated by distances of between 54 and 10692 bp was determined. The results show that in intragenic crosses there is a linear relationship between the recombination frequency observed and distances between the mutations of up to 700 bp. However, no length dependence of the recombination frequency in intergenic crosses with a distance between mutations of 328 to greater than 10000 bp is observed. We attribute this lack of dependence to the high rate of viral DNA interchange, which makes some step other than the cross-over event rate-limiting. Furthermore, we believe that the observed difference in recombination frequency between inter- and intragenic recombination is due to complementation between temperature-sensitive mutants at the permissive temperature.
测定了痘苗病毒突变体之间距离在54至10692 bp之间的完整双因子杂交的重组频率。结果表明，在基因内杂交中，观察到的重组频率与突变之间距离达700 bp时呈线性关系。然而，在突变之间距离为328至大于10000 bp的基因间杂交中，未观察到重组频率的长度依赖性。我们将这种缺乏依赖性归因于病毒DNA交换的高频率，这使得除了交叉事件之外的某些步骤成为限速步骤。此外，我们认为基因间和基因内重组之间观察到的重组频率差异是由于温度敏感突变体在允许温度下的互补作用。
