Chernos V I, Belanov E F, Vasilieva N N
Acta Virol. 1978 Mar;22(2):81-90.
Fifty-seven temperature-sensitive (ts) mutants of a neurotropic variant of vaccinia virus were obtained. A comparison of the effectiveness of 6 different mutagens showed that in in vivo experiments nitrosomethylurea induced 10%, nitrosoguanidine 8%, and bromodeoxyuridine 2.5% ts mutants. In vitro, at the same degree of inactivation, UV irradiation, hydroxylamine and nitrous acid induced 2.5% ts mutants. Out of 49 mutants studied, 27 had a DNA+ phenotype, 19 a DNA+/-and 3 a DNA- phenotype. Under permissive conditions, two thirds of all the mutants tested formed thermolabile virions. In experiments on genetic recombination, the recombination frequency between different pairs was from 2.8 to 57%. Seven of the mutants were plotted on the virus genetic map in a certain sequence.
获得了痘苗病毒神经嗜性变体的57个温度敏感（ts）突变体。对6种不同诱变剂的效果比较表明，在体内实验中，亚硝基甲基脲诱导产生10%的ts突变体，亚硝基胍诱导产生8%，溴脱氧尿苷诱导产生2.5%。在体外，在相同失活程度下，紫外线照射、羟胺和亚硝酸诱导产生2.5%的ts突变体。在研究的49个突变体中，27个具有DNA+表型，19个具有DNA+/-表型，3个具有DNA-表型。在允许条件下，所有测试突变体的三分之二形成热不稳定病毒粒子。在基因重组实验中，不同对之间的重组频率为2.8%至57%。其中7个突变体按一定顺序绘制在病毒基因图谱上。
