Eftekhari Fatemeh, Escobedo Carlos, Ferreira Jacqueline, Duan Xiaobo, Girotto Emerson M, Brolo Alexandre G, Gordon Reuven, Sinton David
Electrical and Computer Engineering, University of Victoria, Victoria, British Columbia, Canada.
Anal Chem. 2009 Jun 1;81(11):4308-11. doi: 10.1021/ac900221y.
We combine nanofluidics and nanoplasmonics for surface-plasmon resonance (SPR) sensing using flow-through nanohole arrays. The role of surface plasmons on resonant transmission motivates the application of nanohole arrays as surface-based biosensors. Research to date, however, has focused on dead-ended holes, and therefore failed to harness the benefits of nanoconfined transport combined with SPR sensing. The flow-through format enables rapid transport of reactants to the active surface inside the nanoholes, with potential for significantly improved time of analysis and biomarker yield through nanohole sieving. We apply the flow-through method to monitor the formation of a monolayer and the immobilization of an ovarian cancer biomarker specific antibody on the sensing surface in real-time. The flow-through method resulted in a 6-fold improvement in response time as compared to the established flow-over method.
我们将纳米流体学和纳米等离子体学相结合，利用流通式纳米孔阵列进行表面等离子体共振（SPR）传感。表面等离子体在共振传输中的作用推动了纳米孔阵列作为基于表面的生物传感器的应用。然而，迄今为止的研究都集中在死端孔上，因此未能利用纳米受限传输与SPR传感相结合的优势。流通式设计能够使反应物快速传输到纳米孔内的活性表面，通过纳米孔筛分有可能显著缩短分析时间并提高生物标志物产量。我们应用流通式方法实时监测传感表面上单层的形成以及卵巢癌生物标志物特异性抗体的固定化。与已确立的流通式方法相比，流通式方法使响应时间缩短了6倍。
