Fenoradosoa T A, Delattre C, Laroche C, Wadouachi A, Dulong V, Picton L, Andriamadio P, Michaud P
Laboratoire de Génie Chimique et Biochimique, Université Blaise Pascal, Polytech Clermont-Ferrand, 24 Avenue des Landais, 63174 Aubière Cedex, France.
Int J Biol Macromol. 2009 Aug 1;45(2):140-5. doi: 10.1016/j.ijbiomac.2009.04.015. Epub 2009 May 3.
Halymenia durvillei is a red seaweed with a great potential as sulphated galactan producer collected in the coastal waters of small island of Madagascar (Nosy-be in Indian Ocean). To elucidate the structure of its polysaccharide, NMR (1H and 13C), FTIR, HPAEC and different colorimetric methods were carried out. It has been shown that this polysaccharide, consisted mainly of galactose, was branched by xylose and galactose in minor amounts. Arabinose and fucose were also detected. This galactan was found highly sulphated (42%, w/w) and pyruvylated (1.8%, w/w). Analysis of glycosidic linkages by CPG-MS and 13C NMR indicated that the polysaccharide has the defining linear backbone of alternating 3-beta-D-galactopyranosyl units and 4-linked alpha-L/D-galactopyranosyl residues. 3,6-Anhydrogalactose units have been also detected in minor quantity. This lambda-carrageenan like polysaccharide has shown original sulphatation patterns with 2-O (26%) or 2/6-O (58%) sulphated 3-linked beta-D-galactopyranosyl units and 6-O (19%) or 2/6-O (47%) 4-linked alpha-L/D-galactopyranosyl residues.
杜氏海膜是一种红藻，在马达加斯加小岛（印度洋的诺西贝）沿海水域采集的作为硫酸化半乳聚糖生产者具有巨大潜力。为了阐明其多糖的结构，进行了核磁共振（1H和13C）、傅里叶变换红外光谱、高效阴离子交换色谱和不同的比色法。结果表明，这种多糖主要由半乳糖组成，少量被木糖和半乳糖分支。还检测到阿拉伯糖和岩藻糖。这种半乳聚糖被发现高度硫酸化（42%，w/w）和丙酮酸化（1.8%，w/w）。通过毛细管气相色谱-质谱联用和13C核磁共振对糖苷键的分析表明，该多糖具有交替的3-β-D-吡喃半乳糖基单元和4-连接的α-L/D-吡喃半乳糖基残基的确定线性主链。还检测到少量的3,6-脱水半乳糖单元。这种类似λ-卡拉胶的多糖显示出原始的硫酸化模式，具有2-O（26%）或2/6-O（58%）硫酸化的3-连接β-D-吡喃半乳糖基单元和6-O（19%）或2/6-O（47%）4-连接α-L/D-吡喃半乳糖基残基。
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