大鼠结状神经节神经元中ATP激活电流的特性
Characteristics of ATP-activated current in nodose ganglion neurons of rats.
作者信息
Tan Yan, Zhao Bin, Zeng Qing-Chun, Shi Chun-Mei, Zhao Feng-Bo, Li Zhi-Wang
机构信息
Department of Neurology, The Affiliated Hospital of Guangdong Medical College, No. 57, People's Avenue South, Xiashan, Zhanjiang 524001, China.
出版信息
Neurosci Lett. 2009 Jul 31;459(1):25-9. doi: 10.1016/j.neulet.2009.04.054. Epub 2009 May 4.
The characteristics of ATP-activated currents (I(ATP)) in rat nodose ganglion (NG) neurons have not been fully clarified. Especially, the correlation between I(ATP) phenotype and P2X receptor subunit genotype in rat NG neuron is not clear. By whole-cell patch-clamp and single cell immunocytochemical techniques, we explored the characteristics of the I(ATP) phenotype and its correlation with P2X receptor subunits in acutely isolated NG neuron of rats. The results indicated that I(ATP) of NG neurons can be classified into four types: F type (fast type, 8.1%), I type (intermediate type, 14.8%), S type (slowing type, 37.0%) and vS type (very slowing type, 21.5%). The single immunocytochemical studies have demonstrated that F type cells express P2X1 and P2X3 subunits, I type cells P2X1, P2X3 and P2X4 subunits, S type cells P2X1, P2X2 and P2X3 subunits and vS type cells P2X2, P2X3 and P2X4 subunits. The results reveal that there are four types of I(ATP) in NG neurons, differential expression of distinct P2X subunits may underlie the I(ATP) phenotype.
大鼠结状神经节（NG）神经元中ATP激活电流（I(ATP)）的特性尚未完全阐明。特别是，大鼠NG神经元中I(ATP)表型与P2X受体亚基基因型之间的相关性尚不清楚。通过全细胞膜片钳和单细胞免疫细胞化学技术，我们探讨了急性分离的大鼠NG神经元中I(ATP)表型的特征及其与P2X受体亚基的相关性。结果表明，NG神经元的I(ATP)可分为四种类型：F型（快速型，8.1%）、I型（中间型，14.8%）、S型（缓慢型，37.0%）和vS型（非常缓慢型，21.5%）。单细胞免疫细胞化学研究表明，F型细胞表达P2X1和P2X3亚基，I型细胞表达P2X1、P2X3和P2X4亚基，S型细胞表达P2X1、P2X2和P2X3亚基，vS型细胞表达P2X2、P2X3和P2X4亚基。结果显示，NG神经元中存在四种类型的I(ATP)，不同P2X亚基的差异表达可能是I(ATP)表型的基础。
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