Mizuno Takuya, Kanbayashi Satoshi, Okawa Takumi, Maeda Sadatoshi, Okuda Masaru
Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
Vet Immunol Immunopathol. 2009 Sep 15;131(1-2):140-3. doi: 10.1016/j.vetimm.2009.03.014. Epub 2009 Mar 31.
The newly discovered cytokine, interleukin-31 (IL-31), belongs to the short-chain cytokine group. It was reported that transgenic expression of IL-31-induced pruritus, similar to atopic dermatitis, in mice, further, excessive amounts of IL-31 was also expressed in the skin from human patients with atopic dermatitis as compared to that from normal people. In this study, canine IL-31 was molecularly cloned from concanavalin A-stimulated canine peripheral blood mononuclear cells (PBMCs), and its nucleotide sequence was determined. Canine IL-31 contains 4 alpha-helix structures characteristic of the IL-31 family, and the amino acid identity of canine IL-31 with those of human or mouse is 54% and 28%, respectively. Furthermore, we detected low levels of canine IL-31 in the thymus, testis, spleen, and kidneys, but not in the skin of atopic dogs.
新发现的细胞因子白细胞介素-31（IL-31）属于短链细胞因子家族。据报道，IL-31在小鼠中的转基因表达会诱发瘙痒，类似于特应性皮炎，此外，与正常人相比，特应性皮炎患者皮肤中也表达过量的IL-31。在本研究中，从刀豆蛋白A刺激的犬外周血单个核细胞（PBMC）中分子克隆犬IL-31，并测定其核苷酸序列。犬IL-31含有IL-31家族特有的4个α-螺旋结构，犬IL-31与人及小鼠IL-31的氨基酸同一性分别为54%和28%。此外，我们在胸腺、睾丸、脾脏和肾脏中检测到低水平的犬IL-31，但在特应性犬的皮肤中未检测到。
