Maeda Sadatoshi, Tsukui Toshihiro, Saze Koh-ichi, Masuda Kenichi, Ohno Koichi, Tsujimoto Hajime, Iwabuchi Shigehiro
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Vet Immunol Immunopathol. 2005 Jan 10;103(1-2):83-92. doi: 10.1016/j.vetimm.2004.08.021.
A monoclonal antibody to canine thymus and activation-regulated chemokine (TARC/CCL17) was developed to examine the association of TARC with the immunopathogenesis of canine atopic dermatitis (AD). Recombinant canine TARC was prepared using an E. coli expression system. Results of transwell chemotaxis assay demonstrated that the recombinant canine TARC showed chemotactic activity for canine lymphoid cells expressing CC chemokine receptor 4 (CCR4). Mice were then immunized with the recombinant canine TARC to obtain monoclonal antibodies. Among the monoclonal antibodies thereby obtained, one monoclonal antibody (CTA-1) was found to react with both recombinant and authentic canine TARC in ELISA and flowcytometric assays, respectively. Immunohistochemical analysis using the monoclonal antibody CTA-1 demonstrated that keratinocytes were major TARC producing cells in lesional skin of dogs with AD.
为研究犬胸腺和激活调节趋化因子（TARC/CCL17）与犬特应性皮炎（AD）免疫发病机制的关联，制备了一种针对犬TARC的单克隆抗体。使用大肠杆菌表达系统制备重组犬TARC。Transwell趋化试验结果表明，重组犬TARC对表达CC趋化因子受体4（CCR4）的犬淋巴细胞具有趋化活性。然后用重组犬TARC免疫小鼠以获得单克隆抗体。在由此获得的单克隆抗体中，发现一种单克隆抗体（CTA-1）在ELISA和流式细胞术检测中分别与重组犬TARC和天然犬TARC发生反应。使用单克隆抗体CTA-1进行的免疫组织化学分析表明，角质形成细胞是患有AD的犬病变皮肤中产生TARC的主要细胞。
