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外显子连接复合体在多个步骤增强剪接后mRNA的翻译。

Exon junction complex enhances translation of spliced mRNAs at multiple steps.

作者信息

Lee Hyung Chul, Choe Junho, Chi Sung-Gil, Kim Yoon Ki

机构信息

School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.

出版信息

Biochem Biophys Res Commun. 2009 Jul 3;384(3):334-40. doi: 10.1016/j.bbrc.2009.04.123. Epub 2009 May 3.

DOI:10.1016/j.bbrc.2009.04.123
PMID:19409878
Abstract

Translation of spliced mRNAs is enhanced by exon junction complex (EJC), which is deposited on mRNAs as a result of splicing. Although this phenomenon itself is well known, the underlying molecular mechanism remains poorly understood. Here we show, using siRNAs against Y14 and eIF4AIII and spliced or intronless constructs that contain different types of internal ribosome entry sites (IRESes), that Y14 and eIF4AIII increase translation of spliced mRNAs before and after formation of the 80S ribosome complex, respectively. These results suggest that EJC modulates translation of spliced mRNA at multiple steps.

摘要

外显子连接复合体（EJC）可增强剪接后mRNA的翻译，该复合体在剪接过程中沉积于mRNA上。尽管这一现象本身广为人知，但其潜在的分子机制仍知之甚少。在此，我们使用针对Y14和eIF4AIII的小干扰RNA（siRNA）以及含有不同类型内部核糖体进入位点（IRES）的剪接或无内含子构建体表明，Y14和eIF4AIII分别在80S核糖体复合体形成之前和之后增加剪接后mRNA的翻译。这些结果表明，EJC在多个步骤调节剪接后mRNA的翻译。

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