Itoh Kenji, Hiromori Youhei, Kato Naoko, Yoshida Ichiro, Itoh Norio, Ike Michihiko, Nagase Hisamitsu, Tanaka Keiichi, Nakanishi Tsuyoshi
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Gen Comp Endocrinol. 2009 Sep 15;163(3):285-91. doi: 10.1016/j.ygcen.2009.04.025. Epub 2009 May 3.
We investigated the effects of retinoic acids (RAs) on steroid hormone production and mRNA expression of steroidogenic enzymes in rat placenta in vitro and in vivo. In the rat trophoblast giant cell line Rcho-1, the natural retinoid X receptor (RXR) agonist 9-cis retinoic acid (9cRA) and synthetic RXR agonist LG100268 slightly promoted production of progesterone and androgen, whereas the natural retinoic acid receptor (RAR) agonist all-trans retinoic acid (atRA) and synthetic RAR agonist TTNPB did not. Furthermore, although administration of atRA and 9cRA into the rat uterus at 13.5days postcoitum robustly induced mRNA expression of cellular retinol binding protein II, the gene for which is targeted by RAR and/or RXR, in the placenta, neither RA affected the expression of placental steroidogenic enzymes, and both had little effect on progesterone and androgen levels in the placenta and embryo, suggesting that placental steroidogenesis is not regulated by RAs in rats.
我们在体外和体内研究了视黄酸（RAs）对大鼠胎盘类固醇激素生成及类固醇生成酶mRNA表达的影响。在大鼠滋养层巨细胞系Rcho-1中，天然类视黄醇X受体（RXR）激动剂9-顺式视黄酸（9cRA）和合成RXR激动剂LG100268可轻微促进孕酮和雄激素的生成，而天然视黄酸受体（RAR）激动剂全反式视黄酸（atRA）和合成RAR激动剂TTNPB则无此作用。此外，虽然在妊娠13.5天向大鼠子宫内注射atRA和9cRA可强烈诱导胎盘细胞视黄醇结合蛋白II的mRNA表达（该基因是RAR和/或RXR的靶基因），但两种视黄酸均不影响胎盘类固醇生成酶的表达，且对胎盘和胚胎中的孕酮和雄激素水平影响甚微，这表明大鼠胎盘类固醇生成不受视黄酸调控。
