Wang Lei, Jiang Dian-Ming
Department of Orthopedics, First Affiliated Hospital, Chongqing Medical University, #1 Youyi Rd, Chongqing 400016, China. wlei
J Ethnopharmacol. 2009 Jul 15;124(2):219-23. doi: 10.1016/j.jep.2009.04.045. Epub 2009 May 3.
The aim of this study was to investigate the protective effect of Buyang Huanwu Decoction, a traditional Chinese medicine formula, on spinal ischemia/reperfusion injury and explore the possible mechanism of the protective effect.
The spinal ischemia/reperfusion injury model was conducted in male Sprague-Dawley rats, and 40 g/kg Buyang Huanwu Decoction was administered by introgastric infusion. Motor function of hind limbs and apoptosis index were measured 72 h after reperfusion was started. The expression of thioredoxin and thioredoxin reductase was examined at 6h and at 24h after reperfusion.
Motor function scores and apoptosis indices were significantly improved in the Buyang Huanwu Decoction group, as compared to the saline-infused control group. Spinal ischemia/reperfusion injury resulted in a decrease in the expression of thioredoxin, while Buyang Huanwu Decoction administration greatly elevated the expression of thioredoxin-1/thioredoxin-2 mRNA and thioredoxin reductase-1/thioredoxin reductase-2 mRNA.
Our results suggest that administration of Buyang Huanwu Decoction may reduce spinal ischemia/reperfusion damage. This neuroprotective effect may be mediated, in part, by an increase in the transcription of thioredoxin.
本研究旨在探讨中药补阳还五汤对脊髓缺血/再灌注损伤的保护作用，并探究其保护作用的可能机制。
在雄性Sprague-Dawley大鼠中建立脊髓缺血/再灌注损伤模型，通过灌胃给予40 g/kg补阳还五汤。在再灌注开始72小时后测量后肢运动功能和凋亡指数。在再灌注后6小时和24小时检测硫氧还蛋白和硫氧还蛋白还原酶的表达。
与生理盐水灌注对照组相比，补阳还五汤组的运动功能评分和凋亡指数显著改善。脊髓缺血/再灌注损伤导致硫氧还蛋白表达降低，而给予补阳还五汤可显著提高硫氧还蛋白-1/硫氧还蛋白-2 mRNA和硫氧还蛋白还原酶-1/硫氧还蛋白还原酶-2 mRNA的表达。
我们的结果表明，给予补阳还五汤可能减轻脊髓缺血/再灌注损伤。这种神经保护作用可能部分是通过硫氧还蛋白转录增加介导的。
