Liu Xiyao, Min Yue, Gu Weiwang, Wang Yujue, Tian Yuguang
Department of Neurosurgery, First Affiliated Hospital of Xiamen University Xiamen 361003, China.
Department of Laboratory Animal Center, Southern Medical University Guangzhou 510515, China.
Int J Clin Exp Med. 2015 Oct 15;8(10):18448-54. eCollection 2015.
Neonatal hypoxic-ischemic encephalopathy (HIE) is a clinical syndrome manifested by neurological symptoms in the first days of life in term infants.
To investigate the therapy effect of Buyanghuanwu Tang (BYHWT), a decoction with 7 herbal ingredients, on neonatal rats with hypoxic ischemic encephalopathy (HIE) and its mechanism.
50 3-week male Sprague-Dawley rats were divided into normal control group, model group, BYHWT 1d group, BYHWT 3d group and BYHWT 7d group, 10 rats in each group. The HIE model of was established in later 4 groups. The later 3 groups were treated with BYHWT for 1, 3 and 7 days, respectively, and the normal control group and model group were treated with PBS. The Morris water maze test and dynamic (18)F-FDG-PET/CT imaging were performed. The changes of hippocampal tissue observed by histopathologic examination, and the expressions of JNK1/JNK2 and TNF-α protein were observed western blotting.
Compared with model group, the impaired performance on distance and latency parameters was mitigated in BYHWT 1d group, BYHWT 3d group and BYHWT 7d group (P < 0.01), the FDG uptake was decreased in BYHWT 3d group and BYHWT 7d group, the apoptotic cells and inflammatory cells were significantly decreased in BYHWT 3d group and BYHWT 7d group, and the expressions of JNK1/JNK2 and TNF-α protein were significantly decreased in BYHWT 7d group (P < 0.05).
BYHWT can delay the HIE onset and preserve the motor function, primarily by regulating inflammation, apoptosis and inhibition by mediating JNK signaling.
新生儿缺氧缺血性脑病（HIE）是足月儿出生后最初几天出现神经症状的临床综合征。
研究由7味中药组成的补阳还五汤（BYHWT）对缺氧缺血性脑病（HIE）新生大鼠的治疗作用及其机制。
将50只3周龄雄性Sprague-Dawley大鼠分为正常对照组、模型组、补阳还五汤1天组、补阳还五汤3天组和补阳还五汤7天组，每组10只。后4组建立HIE模型。后3组分别用补阳还五汤治疗1、3和7天，正常对照组和模型组用PBS治疗。进行Morris水迷宫试验和动态（18）F-FDG-PET/CT成像。通过组织病理学检查观察海马组织变化，采用蛋白质印迹法观察JNK1/JNK2和TNF-α蛋白表达。
与模型组相比，补阳还五汤1天组、补阳还五汤3天组和补阳还五汤7天组在距离和潜伏期参数上的受损表现得到缓解（P<0.01），补阳还五汤3天组和补阳还五汤7天组的FDG摄取降低，补阳还五汤3天组和补阳还五汤7天组的凋亡细胞和炎性细胞明显减少，补阳还五汤7天组的JNK1/JNK2和TNF-α蛋白表达明显降低（P<0.05）。
补阳还五汤可延缓HIE发病并保留运动功能，主要通过调节炎症、凋亡并通过介导JNK信号通路发挥抑制作用。
