Wang Ya-Yun, Wei Yan-Yan, Huang Jing, Wang Wei, Tamamaki Nobuaiki, Li Yun-Qing, Wu Sheng-Xi
Department of Anatomy and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, People's Republic of China.
J Chem Neuroanat. 2009 Sep;38(1):75-81. doi: 10.1016/j.jchemneu.2009.04.003. Epub 2009 May 3.
5-HT plays an important role in GABA transmission at the spinal cord level. The main purpose of the present article is to find out, which 5-HT receptor subtypes may be involved in the regulation of GABAergic transmission in the spinal dorsal horn, by using the combination of tissue RT-PCR, single-cell RT-PCR and double immunofluorescent staining on the GAD(67)-GFP knock-in mice. The present result showed that 5-HT(1A) receptor subtype mRNA was present on about half of GFP-positive GABAergic interneurons in the spinal dorsal horn, which suggests a role of 5-HT(1A) receptor in GABA-mediated modulation of nociception at the spinal cord level. However, it is unexpected that just a very limited sub-population of the total GABAergic interneurons express 5-HT(2A) receptor mRNA or proteins in the spinal dorsal horn, which suggests that the 5-HT(2A) receptor subtype may not be important for spinal GABAergic effects in nociceptive modulation.
5-羟色胺（5-HT）在脊髓水平的γ-氨基丁酸（GABA）传递中发挥重要作用。本文的主要目的是通过对GAD(67)-GFP基因敲入小鼠进行组织逆转录聚合酶链反应（RT-PCR）、单细胞RT-PCR和双重免疫荧光染色相结合的方法，找出哪些5-HT受体亚型可能参与脊髓背角GABA能传递的调节。目前的结果表明，5-HT(1A)受体亚型mRNA存在于脊髓背角约一半的绿色荧光蛋白（GFP）阳性GABA能中间神经元上，这表明5-HT(1A)受体在脊髓水平GABA介导的伤害性感受调节中发挥作用。然而，出乎意料的是，在脊髓背角中，仅总GABA能中间神经元的一个非常有限的亚群表达5-HT(2A)受体mRNA或蛋白，这表明5-HT(2A)受体亚型可能对脊髓GABA能在伤害性感受调节中的作用并不重要。
