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使用旋光测定法检测和鉴定旋光性——在生物光子学、生物医学和生物化学中的应用。

Detection and identification of optical activity using polarimetry--applications to biophotonics, biomedicine and biochemistry.

作者信息

Bahar Ezekiel

机构信息

Electrical Engineering Department, University of Nebraska-Lincoln, Lincoln, NE 68588-0511, USA.

出版信息

J Biophotonics. 2008 Aug;1(3):230-7. doi: 10.1002/jbio.200810021.

DOI:10.1002/jbio.200810021
PMID:19412972
Abstract

Polarized light that is reflected or transmitted through chiral specimens can be used to detect and identify biological and chemical materials including human tissue. The determination of the silent footprints of the chiral properties of the biological materials on scattered polarized light is the basis for these investigations. It is of primary importance to identify which combinations of the elements of the Mueller matrix for reflected or for transmitted light can be used to determine the optical activity of the biochemical materials. The optical activity of chiral materials is characterized by optical rotation and circular dichroism. The explicit analytical dependence of these specific elements of the Mueller matrix, upon the angles of incidence and scatter, upon the wavelength and upon the type of chirality has the potential to provide experimentalists with guidance in determining the optimum use of optical polarimetric scatterometers.

摘要

通过手性样本反射或透射的偏振光可用于检测和识别包括人体组织在内的生物和化学物质。确定生物材料手性特性在散射偏振光上的无声印记是这些研究的基础。识别用于反射光或透射光的穆勒矩阵元素的哪些组合可用于确定生化材料的旋光性至关重要。手性材料的旋光性由旋光和圆二色性表征。穆勒矩阵的这些特定元素对入射角和散射角、波长以及手性类型的明确解析依赖性,有可能为实验人员在确定光学偏振散射仪的最佳用途时提供指导。

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