Ueda Hiroto, Shinoda Tetsuro, Hiruma Kiyoshi
Faculty of Agriculture and Life Sciences, Hirosaki University, Hirosaki 036-8561, Japan.
J Insect Physiol. 2009 Sep;55(9):798-804. doi: 10.1016/j.jinsphys.2009.04.013. Epub 2009 May 18.
The developmental expressions of the mRNA of JH synthetic enzymes have been studied using homogenates of the corpora cardiaca-corpora allata (CC-CA) complexes in Bombyx mori [Kinjoh, T., Kaneko, Y., Itoyama, K., Mita, K., Hiruma, K., Shinoda, T., 2007. Control of juvenile hormone biosynthesis in Bombyx mori: cloning of the enzymes in the mevalonate pathway and assessment of their developmental expression in the corpora allata. Insect Biochemistry and Molecular Biology 37, 808-818]. The in situ hybridization analyses in the CC-CA complex showed that the distribution of the mRNAs of all the mevalonate enzymes and juvenile hormone (JH) acid O-methyltransferase occurred only in the CA cells, indicating that the fluctuations of the enzyme mRNA amounts in the CC-CA complexes were derived solely from the CA. In addition, the size of the CA and their nuclei was not associated with the JH synthetic activity by the CA until the pharate adult. Only female adult CA synthesized JH in B. mori, and the CA and the nuclei were significantly larger than those of male CA which do not synthesize JH.
利用家蚕心侧体-咽侧体（CC-CA）复合体的匀浆，研究了保幼激素合成酶mRNA的发育表达情况[Kinjoh, T., Kaneko, Y., Itoyama, K., Mita, K., Hiruma, K., Shinoda, T., 2007。家蚕保幼激素生物合成的调控：甲羟戊酸途径中酶的克隆及其在咽侧体中的发育表达评估。昆虫生物化学与分子生物学37, 808 - 818]。CC-CA复合体的原位杂交分析表明，所有甲羟戊酸酶和保幼激素（JH）酸O-甲基转移酶的mRNA仅分布在咽侧体细胞中，这表明CC-CA复合体中酶mRNA量的波动仅源于咽侧体。此外，在化蛹成虫之前，咽侧体及其细胞核的大小与咽侧体的保幼激素合成活性无关。在家蚕中，只有雌成虫的咽侧体合成保幼激素，并且其咽侧体和细胞核明显大于不合成保幼激素的雄虫咽侧体。
