Ren Hong-Lin, Xu Dan-Dan, Gopalakrishnan Singaram, Qiao Kun, Huang Wei-Bin, Wang Ke-Jian
State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science, Xiamen University, Xiamen, China.
Dev Comp Immunol. 2009 Sep;33(9):980-90. doi: 10.1016/j.dci.2009.04.003. Epub 2009 May 14.
Glutathione S-transferases (GSTs) are a multigene family of xenobiotic metabolizing phase II detoxification enzymes which take part in many pathological and physiological processes, and which can potentially be used as indicators and biomarkers for cancer diagnoses and organic or inorganic pollutant exposure. In this study, a full-length cDNA of a sigma class GST (abGSTsigma) (GenBank accession number EF546619) from variously colored abalone (Haliotis diversicolor) was identified. It was 1328bp containing an open reading frame of 624bp, encoding 208 amino acid residues with a predicted protein molecular weight of 23.67kDa and an estimated pI of 5.67. Sequence analysis showed that the predicted protein sequence of abGSTsigma cDNA contained the conserved domain of the GST_N_Sigma_like (PSSM: cd03039) and GST_C_Sigma_like (PSSM: cd03192). Alignment analysis demonstrated that the abGSTsigma of H. diversicolor was in a branch position with other known class sigma GSTs from different organisms. The abGSTsigma mRNA was distributed in multiple tissues tested and was highly demonstrated in the gill and mantle of normal abalones. In bacteria-challenged abalone, the abGSTsigma gene was significantly expressed in the hemocytes, gill, mantle and digestive gland and the total GSTs enzyme and SOD were also induced in the four tissues. The increased activities of SOD and GSTs can result in the elimination of reactive oxygen species (ROS) indicating antioxidant activities involved. The preliminary work revealed that the sigma class glutathione S-transferase gene abGSTsigma, a phase II detoxification enzyme, had a positive response to bacterial challenge, and that will lead to an insightful study on elucidating the interactions between immune responses and biotransformation exerted by abGSTsigma.
谷胱甘肽S-转移酶（GSTs）是一个参与多种病理和生理过程的外源性物质代谢II期解毒酶的多基因家族，并且有可能用作癌症诊断以及有机或无机污染物暴露的指标和生物标志物。在本研究中，从不同颜色的皱纹盘鲍（Haliotis diversicolor）中鉴定出了一个sigma类GST（abGSTsigma）的全长cDNA（GenBank登录号EF546619）。它为1328bp，包含一个624bp的开放阅读框，编码208个氨基酸残基，预测的蛋白质分子量为23.67kDa，估计的pI为5.67。序列分析表明，abGSTsigma cDNA的预测蛋白质序列包含GST_N_Sigma_like（PSSM：cd03039）和GST_C_Sigma_like（PSSM：cd03192）的保守结构域。比对分析表明，皱纹盘鲍的abGSTsigma与来自不同生物体的其他已知sigma类GST处于一个分支位置。abGSTsigma mRNA分布于所检测的多个组织中，在正常鲍鱼的鳃和外套膜中高度表达。在受到细菌攻击的鲍鱼中，abGSTsigma基因在血细胞、鳃、外套膜和消化腺中显著表达，并且这四个组织中的总GSTs酶和SOD也被诱导。SOD和GSTs活性的增加可导致活性氧（ROS）的消除，表明涉及抗氧化活性。初步工作表明，作为II期解毒酶的sigma类谷胱甘肽S-转移酶基因abGSTsigma对细菌攻击有阳性反应，这将为阐明abGSTsigma所发挥的免疫反应与生物转化之间的相互作用带来有深刻见解的研究。
