Wilmes Paul, Bond Philip L
Department of Earth and Planetary Science, University of California at Berkeley, Berkeley, CA 94720, USA.
Curr Opin Microbiol. 2009 Jun;12(3):310-7. doi: 10.1016/j.mib.2009.03.004. Epub 2009 May 4.
Molecular techniques are providing unprecedented insights into the organismal and functional make-up of natural microbial consortia. Apart from nucleic acid based approaches, community proteomics has the potential to provide a high-resolution representation of genotypic and phenotypic traits of distinct community members. With the recent availability of extensive genomic sequences from different microbial ecosystems, community proteomics has thus far been applied to activated sludge, acid mine drainage biofilms, freshwater and seawater, soil, symbiotic communities, and gut microbiota. Although these studies differ considerably in the depth of coverage of their respective protein complements, they highlight the power of community proteomics in providing a conclusive link between community composition, physilogy, function, interaction, ecology, and evolution.
分子技术正在为深入了解天然微生物群落的机体组成和功能构成提供前所未有的视角。除了基于核酸的方法外，群落蛋白质组学有潜力提供不同群落成员基因型和表型特征的高分辨率表征。随着近期从不同微生物生态系统获得了大量基因组序列，群落蛋白质组学迄今已应用于活性污泥、酸性矿山排水生物膜、淡水和海水、土壤、共生群落以及肠道微生物群。尽管这些研究在各自蛋白质组覆盖深度上有很大差异，但它们凸显了群落蛋白质组学在提供群落组成、生理学、功能、相互作用、生态学和进化之间的确切联系方面所具有的强大作用。
