Schweder Thomas, Markert Stephanie, Hecker Michael
Institute of Marine Biotechnology, Greifswald, Germany.
Electrophoresis. 2008 Jun;29(12):2603-16. doi: 10.1002/elps.200800009.
Little is known about the life of marine microorganisms under their particular environmental conditions. Genome sequencing combined with the techniques of functional genomics, especially proteome analyses, now open up revolutionary insights into the adaptation strategies marine organisms have evolved in response to the challenges of their habitat. This report summarizes the first approaches and state-of-the-art in the field of proteome analysis of marine bacteria. This includes, amongst others, proteomics on culturable, free-living marine bacteria and on uncultivable bacteria living in symbiosis with higher organisms. Finally, new approaches to determine the metaproteome of uncultured microbial consortia from marine habitats are discussed.
对于海洋微生物在其特定环境条件下的生命活动,我们了解甚少。基因组测序与功能基因组学技术,尤其是蛋白质组分析技术相结合,如今为我们揭示了海洋生物为应对其栖息地挑战而进化出的适应策略,带来了革命性的见解。本报告总结了海洋细菌蛋白质组分析领域的首批方法及最新技术。这其中包括对可培养的、自由生活的海洋细菌以及与高等生物共生的不可培养细菌的蛋白质组学研究。最后,还讨论了用于确定来自海洋栖息地的未培养微生物群落元蛋白质组的新方法。