Borin Sara, Brusetti Lorenzo, Daffonchio Daniele, Delaney Eugenia, Baldi Franco
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, Italy.
Res Microbiol. 2009 Jun;160(5):307-14. doi: 10.1016/j.resmic.2009.04.005. Epub 2009 May 4.
Microbial community structure and diversity in the wide and shallow Venice lagoon were assessed, prior to construction of mobile dams, at nine stations representative of four different sub-basins previously selected on the basis of international guidelines for sediment quality. The sediments were mainly anoxic and were colonized by microbial communities the species richness of which was quantitatively correlated with total elemental sulfur and acid-volatile sulfide. Automated ribosomal intergenic spacer analysis clustered the stations into three groups. One station for each group was hence analyzed in detail for bacterial and archaeal diversity by screening of 16S rRNA gene clone libraries. The dominance of Gammaproteobacteria clones (84% with a high proportion of Vibrionaceae, indicator of urban pollution) determined significant divergence of the station adjacent to industrial and metropolitan areas. Bacteroidetes were widespread, especially where prairies of aquatic plants are located. The other two analyzed stations were dominated by bacterial taxa implicated in the sulfur cycle: the anoxygenic photosynthetic Chromatiales, sulfate- and sulfur-reducing Desulfobacterales and Desulfuromonadales, and members of the Alpha- and Epsilonproteobacteria.
在建造移动水坝之前，根据国际沉积物质量指南，在代表四个不同子流域的九个站点，对宽阔且浅的威尼斯泻湖中的微生物群落结构和多样性进行了评估。沉积物主要处于缺氧状态，被微生物群落定殖，其物种丰富度与总元素硫和酸挥发性硫化物呈定量相关。自动核糖体基因间隔区分析将这些站点聚类为三组。因此，通过筛选16S rRNA基因克隆文库，对每组中的一个站点进行了细菌和古菌多样性的详细分析。γ-变形菌纲克隆占主导地位（84%，其中弧菌科比例很高，是城市污染的指标），这决定了与工业和大都市地区相邻的站点存在显著差异。拟杆菌门分布广泛，尤其是在水生植物草原所在的地方。另外两个分析站点以参与硫循环的细菌类群为主：无氧光合的着色菌目、硫酸盐还原和硫还原的脱硫杆菌目和脱硫单胞菌目，以及α-和ε-变形菌纲的成员。
