在不同钾离子水平下硫胺素焦磷酸对支链α-酮酸脱氢酶激酶的抑制作用
Inhibition of branched-chain alpha-ketoacid dehydrogenase kinase by thiamine pyrophosphate at different potassium ionic levels.
作者信息
Akita Keiichi, Fujimura Yukihiro, Bajotto Gustavo, Shimomura Yoshiharu
机构信息
Department of Materials Science and Engineering, Nagoya Institute of Technology, Nagoya, Japan.
出版信息
Biosci Biotechnol Biochem. 2009 May;73(5):1189-91. doi: 10.1271/bbb.80749. Epub 2009 May 7.
Inhibition of branched-chain alpha-ketoacid dehydrogenase kinase (BDK) by thiamine pyrophosphate (TPP) was analyzed at two potassium ion (K(+)) concentrations. IC(50) values of 4.6 and 8.0 microM and inhibition constant values of 3.2 and 16.4 microM were obtained in the presence of 20 and 100 mM K(+), respectively. These results suggest that BDK is less sensitive to TPP inhibition under physiological TPP and K(+) concentrations.
在两个钾离子（K⁺）浓度下分析了焦磷酸硫胺素（TPP）对支链α-酮酸脱氢酶激酶（BDK）的抑制作用。在分别存在20 mM和100 mM K⁺的情况下，获得的半数抑制浓度（IC₅₀）值分别为4.6和8.0微摩尔，抑制常数分别为3.2和16.4微摩尔。这些结果表明，在生理TPP和K⁺浓度下，BDK对TPP抑制的敏感性较低。
Zotero 插件