Gullotta F, Helpap B
Pathol Eur. 1976;11(4):257-64.
Light and electromicroscopical as well as histochemical investigations were performed on three cases of extraadrenal paragangliomas. They were localized in the carotid body, tympanicum and cauda equina region. Tissue of two cases was cultivated in vitro in nutrient medium TCM 199. The tumours were classified as paragangliomas of the paraganglionic type with typical cell clusters, of the adenomatous and angiomatous type. The enzyme histochemistry showed a very high dehydrogenase activity. Ultrastructurally numerous typical osmiophilic granules could be observed in the cytoplasm of the tumour cells. In tissue culture only a minimal cellular proliferative activity could be detected. The few proliferating cell colonies showed mostly characteristics of epithelial tissue and sometimes a similar behaviour to cells of a ganglioneuroblastoma. The minimal proliferative activity in vitro is in good agreement with the proliferative behaviour of the extraadrenal paragangliomas in vivo.
对三例肾上腺外副神经节瘤进行了光镜、电镜及组织化学研究。肿瘤分别位于颈动脉体、鼓室和马尾区。其中两例的组织在TCM 199培养基中进行了体外培养。这些肿瘤被分类为具有典型细胞簇的副神经节型副神经节瘤、腺瘤型和血管瘤型。酶组织化学显示脱氢酶活性非常高。超微结构上，在肿瘤细胞的细胞质中可观察到大量典型的嗜锇颗粒。在组织培养中仅检测到最小程度的细胞增殖活性。少数增殖细胞集落大多表现出上皮组织的特征，有时表现出与神经母细胞瘤细胞相似的行为。体外最小的增殖活性与肾上腺外副神经节瘤在体内的增殖行为高度一致。
