一项旨在估计乌干达布塔莱贾区牛非洲锥虫病流行率的实地研究。
A field study to estimate the prevalence of bovine African Trypanosomosis in Butaleja District, Uganda.
作者信息
Jing Zhang, Magona Joseph W, Sakurai Tatsuya, Thekisoe Oriel M M, Otim Charles P, Sugimoto Chihiro, Inoue Noboru
机构信息
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Japan.
出版信息
J Vet Med Sci. 2009 Apr;71(4):525-7. doi: 10.1292/jvms.71.525.
Prevalence of bovine trypanosomosis was determined from a total of 203 blood samples collected from Butaleja district, eastern Uganda. All samples were examined by microhematocrit centrifuge test (MHC), PCR and ELISA. ELISA was performed in accordance with the OIE standard procedures using Trypanosoma brucei gambiense procyclic form crude antigens. PCR were utilized to identify the species and the subspecies of trypanosome. The overall prevalence of bovine African trypanosomosis was 8.9% by MHC, and 45.3% by the ELISA. Since substantial number (12 out of 18) of MHC positive samples were negative in the PCR tests, we could not conclude the most epidemic trypanosome species in the studied area. Nevertheless, the PCR results suggests that the most prevalent trypanosome was T. b. brucei (31/203), followed by T. congolense (6/203). In addition, only a few (3/203) mixed infections of T. b. brucei and T. congolense was detected by the PCR. Results obtained from this study indicates that bovine trypanosomosis is endemic in Butaleja district, Uganda.
从乌干达东部布塔莱贾区采集的总共203份血样中测定了牛锥虫病的流行率。所有样本均通过微量血细胞比容离心试验（MHC）、聚合酶链反应（PCR）和酶联免疫吸附测定（ELISA）进行检测。ELISA按照世界动物卫生组织（OIE）的标准程序，使用布氏冈比亚锥虫前循环型粗抗原进行。PCR用于鉴定锥虫的种类和亚种。通过MHC检测，牛非洲锥虫病的总体流行率为8.9%，通过ELISA检测为45.3%。由于大量（18份中的12份）MHC阳性样本在PCR检测中呈阴性，我们无法确定研究区域内最流行的锥虫种类。尽管如此，PCR结果表明最常见的锥虫是布氏锥虫（T. b. brucei，31/203），其次是刚果锥虫（T. congolense，6/203）。此外，通过PCR仅检测到少数（3/203）布氏锥虫和刚果锥虫的混合感染。本研究获得的结果表明，牛锥虫病在乌干达布塔莱贾区呈地方流行。
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