猴空泡病毒40（SV40）DNA的限制性片段Hind F-Eco RI2的核苷酸序列

Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.

作者信息

Contreras R, Volckaert G, Thys F, Van de Voorde A, Fiers W

出版信息

Nucleic Acids Res. 1977 Apr;4(4):1001-14. doi: 10.1093/nar/4.4.1001.

DOI:10.1093/nar/4.4.1001

PMID:194225

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342500/

Abstract

The nucleotide sequence of the SV40 genome region between the Hind K fragment and the Eco RI cleavage site has been determined by a combination of three different approaches : analysis of RNA products obtained by transcription with Escherichia coli DNA dependent RNA polymerase, partial degradations with snake venom exonuclease and base-specific chemical degradation of 5'-terminal labeled restriction fragments. This nucleotide sequence shows only one open reading frame and allows the deduction of a small segment of the amino acid sequence of VP1, the major structural protein.

摘要

通过三种不同方法的组合，已确定了猿猴空泡病毒40（SV40）基因组中位于Hind K片段和Eco RI切割位点之间区域的核苷酸序列：分析用大肠杆菌DNA依赖性RNA聚合酶转录获得的RNA产物、用蛇毒外切核酸酶进行部分降解以及对5'-末端标记的限制性片段进行碱基特异性化学降解。该核苷酸序列仅显示一个开放阅读框，并允许推导主要结构蛋白VP1的一小段氨基酸序列。