猴病毒40型DNA的Hind-F-EcoRI1限制性片段的核苷酸序列（VP1基因的一部分）

Nucleotide sequence of the restriction fragment Hind-F-EcoRI1 of simian-virus-40 DNA (part of the VP1 gene).

作者信息

Contreras R, van de Voorde A, Fiers W

出版信息

Eur J Biochem. 1978 May 16;86(2):317-24. doi: 10.1111/j.1432-1033.1978.tb12313.x.

DOI:10.1111/j.1432-1033.1978.tb12313.x

PMID:207520

Abstract

The nucleotide sequence of the simian virus 40 (SV40) genome region between the cleavage sites for restriction endonucleases EcoRI (map position 0) and HindII (map position 0.05) has been determined mainly by the partial chemical DNA degradation procedure of Maxam and Gilbert. This fragment represents 5.3% of the genome of SV40 and is located in the late region, internally in the VP1 gene. The message strand shows only one open reading frame for translation into protein, which connects to the one for the preceding fragment. On this basis part of the amino acid sequence of the VP1 protein is presented.

摘要

猴病毒40（SV40）基因组中位于限制性内切酶EcoRI切割位点（图谱位置0）和HindII切割位点（图谱位置0.05）之间的核苷酸序列，主要通过Maxam和Gilbert的部分化学DNA降解方法确定。该片段占SV40基因组的5.3%，位于晚期区域，在VP1基因内部。编码链仅显示一个用于翻译成蛋白质的开放阅读框，它与前一个片段的开放阅读框相连。在此基础上，给出了VP1蛋白的部分氨基酸序列。