EcoRI核酸内切酶作用机制的底物依赖性
Substrate dependence of the mechanism of EcoRI endonuclease.
作者信息
Rubin R A, Modrich P
出版信息
Nucleic Acids Res. 1978 Aug;5(8):2991-7. doi: 10.1093/nar/5.8.2991.
Abstract
The mechanism of EcoRI endonuclease is substrate dependent. At 37 degrees dissociation of the enzyme-Form II DNA intermediates of ColE1 DNA and bacteriophage G4 RFI DNA is negligible. Therefore, both DNA strands with in the EcoRI sequence are cleaved during a single binding event. However, double strand cleavage of SV40 DNA occurs without dissociation of the enzyme in only 75% of the catalytic events. This mechanistic difference presumably reflects sequence differences about the EcoRI sites of these DNA's.
摘要
EcoRI核酸内切酶的作用机制取决于底物。在37℃时，ColE1 DNA和噬菌体G4 RFI DNA的酶-形式II DNA中间体的解离可忽略不计。因此，EcoRI序列中的两条DNA链在单次结合事件中都会被切割。然而，SV40 DNA的双链切割仅在75%的催化事件中发生，且酶不会解离。这种机制差异大概反映了这些DNA的EcoRI位点周围的序列差异。
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本文引用的文献
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