磷脂酰胆碱交换蛋白中的脂类-蛋白质相互作用
Lipid-protein interaction in the phosphatidylcholine exchange protein.
作者信息
Devaux P F, Moonen P, Bienvenue A, Wirtz K W
出版信息
Proc Natl Acad Sci U S A. 1977 May;74(5):1807-10. doi: 10.1073/pnas.74.5.1807.
Abstract
Incorporation of 2-acyl spin-labeled lecithin into the phosphatidylcholine protein from bovine liver results in an immobilization of the spin-label at the methyl and the carboxyl terminal end of the acyl chain. The nitroxide group on the protein-bound lecithin molecule is not accessible to ascorbate. This suggests that lecithin is buried in a pocket on the protein, which effectively shields the acyl chains from the medium.
摘要
将2-酰基自旋标记的卵磷脂掺入牛肝磷脂酰胆碱蛋白中，会导致自旋标记固定在酰基链的甲基和羧基末端。与蛋白结合的卵磷脂分子上的氮氧基团无法与抗坏血酸接触。这表明卵磷脂被埋在蛋白质的一个口袋中，有效地将酰基链与介质隔开。
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