Shihabi Zak K
Department of Pathology, Wake Forest University, Baptist Medical Center, Winston-Salem, NC 27157, USA.
Electrophoresis. 2009 May;30(9):1516-21. doi: 10.1002/elps.200800587.
In order to overcome the poor absorbency detection limits in CE, two simple strategies were combined to increase the amount of the sample injected: a long capillary to hold extra sample while leaving adequate room for the separation and acetonitrile stacking, which concentrated the sample based on transient pseudo-ITP. The combination of these two strategies yielded sensitivity comparable or better than that of the HPLC with good separation and with better theoretical plate number. The analysis of mycophenolic acid, a common immunosuppressant drug, in serum was used here as an example to illustrate this enhanced detection and its applicability to therapeutic drug monitoring. Acetonitrile was used to remove serum proteins followed by direct injection filling 5-21% of the capillary volume and separation in a borate buffer. The overall CE method compared well to an assay by HPLC as far as sample preparation, correlation coefficient, and especially sensitivity of detection.
为了克服毛细管电泳中较差的吸收检测限，将两种简单策略相结合以增加进样量：使用长毛细管来容纳额外的样品，同时为分离和乙腈堆积留出足够空间，乙腈堆积基于瞬态假等速电泳浓缩样品。这两种策略的结合产生的灵敏度与高效液相色谱相当或更高，具有良好的分离效果和更高的理论塔板数。本文以血清中常见免疫抑制剂霉酚酸的分析为例，来说明这种增强检测及其在治疗药物监测中的适用性。使用乙腈去除血清蛋白，然后直接进样，填充毛细管体积的5 - 21%，并在硼酸盐缓冲液中进行分离。就样品制备、相关系数，尤其是检测灵敏度而言，整体毛细管电泳方法与高效液相色谱测定法相比表现良好。
