Chen Jun, Wang Feng, Gao Xue, Zha Dingjun, Xue Tao, Cheng Xin, Zhong Cuiping, Han Yu, Qiu Jianhua
Department of Otolaryngology, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi'an, Shaanxi Province 710032, China.
Neurosci Lett. 2009 Apr 10;453(3):166-9. doi: 10.1016/j.neulet.2009.02.019. Epub 2009 Feb 13.
Cyclin A2 plays a major role in cell cycle modulation. Cyclin A2 level has not been determined in the inner ear yet. RT-PCR, Western blotting, immunohistochemistry and immunocytochemistry were used to measure cyclin A2 expression in rat cochlea tissues of different ages, isolated cochlear stem cells and stem cell-derived differentiated cells. The results indicated that cyclin A2 level in cochlea tissues decreased gradually from newborn to adult. Furthermore, cyclin A2 level fell down after differentiation of cochlear stem cells. It was suggested that cyclin A2 might be involved in the modulation of rat cochlea development and cochlear stem cell differentiation.
细胞周期蛋白A2在细胞周期调控中起主要作用。目前尚未测定内耳中的细胞周期蛋白A2水平。采用逆转录聚合酶链反应（RT-PCR）、蛋白质免疫印迹法、免疫组织化学和免疫细胞化学方法，检测不同年龄大鼠耳蜗组织、分离的耳蜗干细胞及干细胞来源的分化细胞中细胞周期蛋白A2的表达。结果表明，从新生到成年，耳蜗组织中的细胞周期蛋白A2水平逐渐降低。此外，耳蜗干细胞分化后，细胞周期蛋白A2水平下降。提示细胞周期蛋白A2可能参与大鼠耳蜗发育及耳蜗干细胞分化的调控。
