Kiba Takayoshi, Kintaka Yuri, Suzuki Yoko, Nakata Eiko, Ishigaki Yasuhito, Inoue Shuji
Laboratory of Clinical Nutrition & Physiology, Kyoritsu Women's University, 2-2-1 Hitotsubashi Chiyoda-ku, Tokyo 101-8433, Japan.
Neurosci Lett. 2009 May 8;455(1):14-6. doi: 10.1016/j.neulet.2009.03.062. Epub 2009 Mar 24.
There are no reports that hypothalamus can directly affect the expression of neuron-related genes and immune-related genes in liver. We identified genes of which expression profiles showed significant modulation in rat liver after ventromedial hypothalamic (VMH) lesions. Total RNA was extracted, and differences in the gene expression profiles between rats at day 3 after VMH lesioning and sham-VMH lesioned rats were investigated using DNA microarray analysis. The result revealed that VMH lesions regulated the genes that were involved in functions related to neuronal development and immunofunction in the liver. Real-time PCR also confirmed that gene expression of SULT4A1 was upregulated, but expression of ACSL1 and CISH were downregulated at day 3 after VMH lesions. VMH lesions may change the expression of neuron-related genes and immune-related genes in rat liver.
尚无报道表明下丘脑可直接影响肝脏中神经元相关基因和免疫相关基因的表达。我们鉴定了在腹内侧下丘脑（VMH）损伤后大鼠肝脏中表达谱显示出显著调节的基因。提取总RNA，并使用DNA微阵列分析研究VMH损伤后第3天的大鼠与假VMH损伤大鼠之间基因表达谱的差异。结果显示，VMH损伤调节了肝脏中与神经元发育和免疫功能相关的基因。实时PCR也证实，VMH损伤后第3天，SULT4A1的基因表达上调，但ACSL1和CISH的表达下调。VMH损伤可能会改变大鼠肝脏中神经元相关基因和免疫相关基因的表达。
