Faculty of Health Care, Kiryu University, 606-7 Azami, Kasakake, Midori, Gunma, 379-2392, Japan.
Neurosci Lett. 2010 Mar 8;471(3):148-51. doi: 10.1016/j.neulet.2010.01.029. Epub 2010 Jan 22.
The mammalian circadian system contains both central and peripheral oscillators. It was reported that the rhythmic expressions of period homolog 2 (per2) mRNA in peripheral tissues were abolished by the suprachiasmatic nucleus (SCN) lesions. However, there are no reports that ventromedial hypothalamic (VMH) lesions can directly affect the expression of clock genes in pancreas and liver. In the present study, we examined whether VMH lesions can affect the expression of the clock gene, per2 in these organs. Total RNA was extracted from rat pancreas and liver tissues, and differences in the gene expression profiles between rats at day 3 after VMH lesioning and sham-VMH-lesioned rats were investigated using DNA microarray and real-time quantitative analysis. These results showed that VMH lesions downregulated the expression of the clock gene, per2 in the pancreas, but not the liver in the morning. There is a possibility that VMH lesions may affect the expression of the clock gene, per2 in the pancreas.
哺乳动物的生物钟系统包含中央和外周振荡器。据报道，外周组织中周期同源物 2 (per2) mRNA 的节律表达被视交叉上核 (SCN) 损伤所消除。然而，目前尚无报道表明腹内侧下丘脑 (VMH) 损伤可以直接影响胰腺和肝脏中时钟基因的表达。在本研究中，我们研究了 VMH 损伤是否会影响这些器官中时钟基因 per2 的表达。从大鼠的胰腺和肝脏组织中提取总 RNA，并使用 DNA 微阵列和实时定量分析研究 VMH 损伤后 3 天大鼠和假 VMH 损伤大鼠之间基因表达谱的差异。这些结果表明，VMH 损伤下调了胰腺中时钟基因 per2 的表达，但在早晨肝脏中没有这种情况。VMH 损伤可能会影响胰腺中时钟基因 per2 的表达。
