Mao Caiping, Wu Jiawei, Xiao Daliao, Lv Juanxiu, Ding Yang, Xu Zhice, Zhang Lubo
Perinatal Biology Center, Soochow University School of Medicine, Suzhou, People's Republic of China.
Reprod Toxicol. 2009 Apr;27(2):149-54. doi: 10.1016/j.reprotox.2009.01.012. Epub 2009 Feb 11.
Maternal cigarette smoking accompanied with fetal and neonatal growth restriction causes abnormalities in organ development in the postnatal life. The present study determined the effect of maternal administration of nicotine on the development of the kidney in rats by examining the expression of renal angiotensin II receptors at mRNA and protein levels as well as kidney weight during postnatal development.
Nicotine was administered to pregnant rats via subcutaneous osmotic minipumps throughout gestation and up to 10 days after delivery. Kidneys were removed and collected from both male and female offspring at ages of 14-day-old, 30-day-old, and 5-month-old. Maternal nicotine administration significantly reduced renal AT(2) receptor (AT(2)R) mRNA and protein abundance in both males and females at all three developmental ages examined.
Although AT(1) receptor (AT(1)R) mRNA and protein levels were not significantly changed between the control offspring and the offspring exposed to maternal nicotine during the early developmental period, the renal AT(1)R/AT(2)R ratio was significantly increased. This was associated with a significant decrease of kidney weight in both male and female offspring.
The results demonstrated that the development of renal angiotensin II receptor could be changed following exposure to perinatal nicotine, and such change in the kidney could be long-term in postnatal life.
母亲吸烟并伴有胎儿和新生儿生长受限会导致出生后器官发育异常。本研究通过检测出生后发育过程中肾血管紧张素II受体在mRNA和蛋白质水平的表达以及肾脏重量，来确定母体给予尼古丁对大鼠肾脏发育的影响。
在整个妊娠期直至分娩后10天，通过皮下渗透微型泵给怀孕大鼠注射尼古丁。在14日龄、30日龄和5月龄时，从雄性和雌性后代中取出并收集肾脏。在所有三个检测的发育年龄阶段，母体给予尼古丁均显著降低了雄性和雌性后代肾脏中AT(2)受体（AT(2)R）的mRNA和蛋白质丰度。
尽管在早期发育阶段，对照后代与暴露于母体尼古丁的后代之间，AT(1)受体（AT(1)R）的mRNA和蛋白质水平没有显著变化，但肾AT(1)R/AT(2)R比值显著增加。这与雄性和雌性后代肾脏重量的显著降低有关。
结果表明，围产期暴露于尼古丁后，肾血管紧张素II受体的发育可能会发生变化，并且这种肾脏变化在出生后可能是长期的。
